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Fitness for 5 genes in
Klebsiella michiganensis M5al
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500 nt
BWI76_RS14130 and BWI76_RS14135 overlap by 4 nucleotides
BWI76_RS14135 and BWI76_RS14140 are separated by 2 nucleotides
BWI76_RS14140 and BWI76_RS14145 are separated by 43 nucleotides
BWI76_RS14145 and BWI76_RS14150 overlap by 17 nucleotides
BWI76_RS14130: BWI76_RS14130 - malto-oligosyltrehalose trehalohydrolase, at 2,952,159 to 2,953,946
_RS14130
BWI76_RS14135: BWI76_RS14135 - malto-oligosyltrehalose synthase, at 2,953,943 to 2,956,483
_RS14135
BWI76_RS14140: BWI76_RS14140 - glycogen debranching enzyme, at 2,956,486 to 2,958,561
_RS14140
BWI76_RS14145: BWI76_RS14145 - dihydrolipoyl dehydrogenase, at 2,958,605 to 2,960,002
_RS14145
BWI76_RS14150: BWI76_RS14150 - dihydrolipoyllysine-residue acetyltransferase, at 2,959,986 to 2,961,515
_RS14150
Group
Condition
BWI76
_RS14130
BWI76
_RS14135
BWI76
_RS14140
BWI76
_RS14145
BWI76
_RS14150
supernatant control
Vogels_fungal_media; pH 5.5
-0.3
-0.4
-0.2
-1.1
-0.8
mixed community
LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
-0.2
-0.5
-0.0
-1.1
-0.2
phage
KLEB17 MOI 1
-0.2
+0.8
+0.3
-2.5
+0.0
phage
KLEB1 MOI 10
+0.0
+0.6
-0.5
-0.7
-0.9
phage
K. M5a1 KLEB22 MOI 10
-0.3
-0.1
-0.3
-1.0
+0.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.3
+0.3
+0.1
-0.7
-0.5
nitrogen source
L-tyrosine (N)
-0.2
+0.5
-0.3
-0.5
-0.4
stress
Fungal_growth_media_mCAFEs_v1 with Zinc 2 mM
+0.3
-0.9
-0.3
-0.3
+0.3
mixed community
RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
+0.6
-1.2
+0.1
-0.6
+0.2
nitrogen source
L-Asparagine (N)
+0.2
-0.8
+0.3
-0.3
-0.3
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.3
+0.3
-0.5
-0.5
+0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.2
+0.8
-0.2
-0.5
-0.6
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
+0.2
-0.9
-0.2
+0.6
-0.1
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB
+0.4
+0.4
+0.1
-0.3
-0.9
phage
KLEB20 MOI 1
-0.6
+0.8
-0.2
-0.4
+0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.4
+0.5
+0.3
+0.1
-0.6
phage
K. M5a1 KLEB23 MOI 10
+0.1
+0.9
-0.3
-1.6
+0.9
stress
Cobalt chloride 0.5 mM
+0.3
+0.6
-0.3
-1.1
+0.7
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.2
+0.9
-0.2
-0.6
+0.3
phage
KLEB29 MOI 1
+0.2
-0.3
+0.1
-0.6
+0.9
mixed community
LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
+0.4
+0.5
+0.2
-0.4
-0.4
phage
K. M5a1 KLEB23 MOI 1
-0.2
+0.3
-0.5
+0.3
+0.5
supernatant
Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
+0.3
-0.7
+0.1
+0.4
+0.4
micoeukaryotes
Dictyostellum sp.mixed culture-1
-0.1
-0.9
+0.5
+1.0
+0.3
phage
KLEB16 MOI 10
+0.1
+0.8
+0.3
-0.6
+0.2
phage
KLEB26 MOI0.1
+0.1
-0.5
+0.3
+0.1
+0.8
phage
KLEB31 MOI 1
+0.3
-0.4
+0.0
+0.3
+1.0
stress
Cobalt chloride 0.5 mM
+0.0
+1.0
-0.1
-0.3
+0.6
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.6
-0.3
+0.2
-0.2
+0.9
phage
K. M5a1 KLEB26 MOI 0.1
-0.1
-0.3
+0.1
+1.2
+0.6
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