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Fitness for 5 genes in
Klebsiella michiganensis M5al
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500 nt
BWI76_RS13110 and BWI76_RS13115 overlap by 4 nucleotides
BWI76_RS13115 and BWI76_RS13120 are separated by 2 nucleotides
BWI76_RS13120 and BWI76_RS13125 are separated by 3 nucleotides
BWI76_RS13125 and BWI76_RS13130 overlap by 11 nucleotides
BWI76_RS13110: BWI76_RS13110 - phenylacetic acid degradation protein, at 2,734,299 to 2,735,369
_RS13110
BWI76_RS13115: BWI76_RS13115 - 2,3-dehydroadipyl-CoA hydratase, at 2,735,366 to 2,736,133
_RS13115
BWI76_RS13120: BWI76_RS13120 - 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase, at 2,736,136 to 2,736,924
_RS13120
BWI76_RS13125: BWI76_RS13125 - 3-hydroxyacyl-CoA dehydrogenase, at 2,736,928 to 2,738,349
_RS13125
BWI76_RS13130: BWI76_RS13130 - phenylacetic acid degradation protein PaaD, at 2,738,339 to 2,738,761
_RS13130
Group
Condition
BWI76
_RS13110
BWI76
_RS13115
BWI76
_RS13120
BWI76
_RS13125
BWI76
_RS13130
phage
K. M5a1 KLEB26 MOI 0.1
+1.2
N.D.
N.D.
-0.9
-5.7
phage
KLEB1 MOI 10
-0.1
N.D.
N.D.
-1.6
-2.0
stress
Sisomicin 0.008 mg/ml
-1.6
N.D.
N.D.
-0.1
-1.2
nitrogen fixation
D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days
-0.8
N.D.
N.D.
-1.1
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-1.0
N.D.
N.D.
-0.9
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-1.1
N.D.
N.D.
-0.7
-0.4
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
+0.5
N.D.
N.D.
-1.3
-1.3
phage
KLEB29 MOI 1
-0.3
N.D.
N.D.
-0.8
-1.0
carbon source
Gly-Glu (C)
-0.5
N.D.
N.D.
-0.2
-1.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-1.0
N.D.
N.D.
-0.2
-0.8
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-1.0
N.D.
N.D.
-0.5
-0.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.1
N.D.
N.D.
-1.7
-0.2
supernatant
Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
-1.5
N.D.
N.D.
+0.2
-0.3
supernatant
Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
-1.3
N.D.
N.D.
+0.5
-0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.4
N.D.
N.D.
-1.2
-0.2
carbon source
L-Sorbose (C)
-1.0
N.D.
N.D.
+0.5
-0.5
mixed community
Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
-0.6
N.D.
N.D.
+0.6
-0.9
phage
KLEB20 MOI 1
-0.6
N.D.
N.D.
-0.8
+0.5
pH
LB, pH9
-0.3
N.D.
N.D.
+0.6
-1.1
supernatant
RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.5 x; and 2,2'-Dipyridyl 50 uM
+0.1
N.D.
N.D.
-1.2
+0.5
supernatant
Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x; and D-Glucose 37 mM
-0.5
N.D.
N.D.
-0.8
+0.7
supernatant
Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
-0.5
N.D.
N.D.
+0.8
-0.8
stress
Fungal_growth_media_mCAFEs_v1 with Zinc 2 mM
-0.6
N.D.
N.D.
+1.0
-0.7
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.7
N.D.
N.D.
-0.5
+0.9
phage
K. M5a1 KLEB23 MOI 10
-0.9
N.D.
N.D.
-0.1
+0.9
phage
KLEB17 MOI 0.1
+0.6
N.D.
N.D.
+0.8
-0.7
ecofab
RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL
+0.8
N.D.
N.D.
-0.5
+1.0
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.5
N.D.
N.D.
-0.1
+1.3
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+2.0
N.D.
N.D.
-0.0
-0.0
phage
K. M5a1 KLEB10 MOI 10
+0.6
N.D.
N.D.
+1.0
+0.8
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