Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS13110 and BWI76_RS13115 overlap by 4 nucleotidesBWI76_RS13115 and BWI76_RS13120 are separated by 2 nucleotidesBWI76_RS13120 and BWI76_RS13125 are separated by 3 nucleotidesBWI76_RS13125 and BWI76_RS13130 overlap by 11 nucleotides BWI76_RS13110: BWI76_RS13110 - phenylacetic acid degradation protein, at 2,734,299 to 2,735,369 _RS13110 BWI76_RS13115: BWI76_RS13115 - 2,3-dehydroadipyl-CoA hydratase, at 2,735,366 to 2,736,133 _RS13115 BWI76_RS13120: BWI76_RS13120 - 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase, at 2,736,136 to 2,736,924 _RS13120 BWI76_RS13125: BWI76_RS13125 - 3-hydroxyacyl-CoA dehydrogenase, at 2,736,928 to 2,738,349 _RS13125 BWI76_RS13130: BWI76_RS13130 - phenylacetic acid degradation protein PaaD, at 2,738,339 to 2,738,761 _RS13130
Group Condition BWI76_RS13110 BWI76_RS13115 BWI76_RS13120 BWI76_RS13125 BWI76_RS13130
phage K. M5a1 KLEB26 MOI 0.1 +1.2 N.D. N.D. -0.9 -5.7
phage KLEB1 MOI 10 -0.1 N.D. N.D. -1.6 -2.0
stress Sisomicin 0.008 mg/ml -1.6 N.D. N.D. -0.1 -1.2
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days -0.8 N.D. N.D. -1.1 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.0 N.D. N.D. -0.9 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.1 N.D. N.D. -0.7 -0.4
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x +0.5 N.D. N.D. -1.3 -1.3
phage KLEB29 MOI 1 -0.3 N.D. N.D. -0.8 -1.0
carbon source Gly-Glu (C) -0.5 N.D. N.D. -0.2 -1.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.0 N.D. N.D. -0.2 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.0 N.D. N.D. -0.5 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 N.D. N.D. -1.7 -0.2
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x -1.5 N.D. N.D. +0.2 -0.3
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml -1.3 N.D. N.D. +0.5 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 N.D. N.D. -1.2 -0.2
carbon source L-Sorbose (C) -1.0 N.D. N.D. +0.5 -0.5
mixed community Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL -0.6 N.D. N.D. +0.6 -0.9
phage KLEB20 MOI 1 -0.6 N.D. N.D. -0.8 +0.5
pH LB, pH9 -0.3 N.D. N.D. +0.6 -1.1
supernatant RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.5 x; and 2,2'-Dipyridyl 50 uM +0.1 N.D. N.D. -1.2 +0.5
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x; and D-Glucose 37 mM -0.5 N.D. N.D. -0.8 +0.7
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x -0.5 N.D. N.D. +0.8 -0.8
stress Fungal_growth_media_mCAFEs_v1 with Zinc 2 mM -0.6 N.D. N.D. +1.0 -0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 N.D. N.D. -0.5 +0.9
phage K. M5a1 KLEB23 MOI 10 -0.9 N.D. N.D. -0.1 +0.9
phage KLEB17 MOI 0.1 +0.6 N.D. N.D. +0.8 -0.7
ecofab RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL +0.8 N.D. N.D. -0.5 +1.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 N.D. N.D. -0.1 +1.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +2.0 N.D. N.D. -0.0 -0.0
phage K. M5a1 KLEB10 MOI 10 +0.6 N.D. N.D. +1.0 +0.8
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