Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS12960 and BWI76_RS12965 are separated by 239 nucleotidesBWI76_RS12965 and BWI76_RS12970 are separated by 111 nucleotidesBWI76_RS12970 and BWI76_RS12975 are separated by 54 nucleotidesBWI76_RS12975 and BWI76_RS12980 are separated by 78 nucleotides BWI76_RS12960: BWI76_RS12960 - transcriptional regulator, at 2,702,062 to 2,702,541 _RS12960 BWI76_RS12965: BWI76_RS12965 - LysR family transcriptional regulator, at 2,702,781 to 2,703,650 _RS12965 BWI76_RS12970: BWI76_RS12970 - MFS transporter, at 2,703,762 to 2,705,198 _RS12970 BWI76_RS12975: BWI76_RS12975 - type I glyceraldehyde-3-phosphate dehydrogenase, at 2,705,253 to 2,706,263 _RS12975 BWI76_RS12980: BWI76_RS12980 - AraC family transcriptional regulator, at 2,706,342 to 2,707,322 _RS12980
Group Condition BWI76_RS12960 BWI76_RS12965 BWI76_RS12970 BWI76_RS12975 BWI76_RS12980
phage KLEB17 MOI 1 N.D. -1.0 -0.5 -0.8 -1.1
nitrogen source L-Arginine (N) N.D. -1.6 +0.1 -1.2 -0.1
phage K. M5a1 KLEB23 MOI 10 N.D. -1.2 -0.2 -0.9 +0.2
phage K. M5a1 KLEB23 MOI 10 N.D. -0.8 -0.0 -0.1 -1.0
phage KLEB24 MOI 1 N.D. -0.8 -0.2 -0.1 -0.7
phage KLEB30 MOI 1 N.D. -1.2 +0.1 -0.8 +0.0
stress Chloramphenicol 0.001 mg/ml N.D. -0.5 +0.1 -0.7 -0.5
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x N.D. -0.5 -0.1 -1.1 +0.1
phage KLEB29 MOI 1 N.D. -1.0 +0.3 -0.4 -0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X N.D. -0.4 +0.2 -0.5 -0.6
phage KLEB1 MOI 10 N.D. -1.4 +0.4 -0.2 -0.2
phage K. M5a1 KLEB26 MOI 0.1 N.D. +0.2 -0.4 -1.2 +0.5
stress Cobalt chloride 0.5 mM N.D. -0.7 +0.3 +0.3 -0.6
phage KLEB1 MOI 0.1 N.D. +0.6 +0.4 -0.4 -1.4
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.6 -0.5 +0.6 +0.1
stress Cobalt chloride 0.5 mM N.D. -0.8 +0.3 -0.4 +0.7
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. +0.1 +0.1 +0.8 -1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.7 +1.1 -0.2 -0.0
nitrogen source L-Serine (N) N.D. -0.9 +0.4 +0.4 +0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. +0.5 -0.2 -0.4 +0.7
phage K. M5a1 KLEB10 MOI 1 N.D. +0.8 +0.1 +0.3 -0.7
mixed community RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x N.D. +1.1 -0.1 -0.5 +0.1
phage KLEB1 MOI 10 N.D. +0.4 -0.6 +0.0 +0.8
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml N.D. +0.8 +0.1 +0.4 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.2 +0.2 +0.6 +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.5 +0.3 +1.1 -0.3
mixed community LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL N.D. +0.7 +0.2 +0.8 -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.5 -0.0 +0.6 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.6 +0.0 +0.6 +0.6
rhizosphere rhizosphere sample 1; outgrowth in LB N.D. -0.0 +0.6 +0.5 +1.3
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