Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS11920 and BWI76_RS11925 are separated by 410 nucleotidesBWI76_RS11925 and BWI76_RS11930 are separated by 329 nucleotidesBWI76_RS11930 and BWI76_RS11935 are separated by 245 nucleotidesBWI76_RS11935 and BWI76_RS11940 are separated by 394 nucleotides BWI76_RS11920: BWI76_RS11920 - hypothetical protein, at 2,507,968 to 2,508,219 _RS11920 BWI76_RS11925: BWI76_RS11925 - DNA topoisomerase I, at 2,508,630 to 2,511,227 _RS11925 BWI76_RS11930: BWI76_RS11930 - LysR family transcriptional regulator CysB, at 2,511,557 to 2,512,531 _RS11930 BWI76_RS11935: BWI76_RS11935 - YmiA family putative membrane protein, at 2,512,777 to 2,512,944 _RS11935 BWI76_RS11940: BWI76_RS11940 - aconitate hydratase 1, at 2,513,339 to 2,516,011 _RS11940
Group Condition BWI76_RS11920 BWI76_RS11925 BWI76_RS11930 BWI76_RS11935 BWI76_RS11940
phage KLEB17 MOI 0.1 -0.1 N.D. N.D. -1.1 -0.6
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM -0.3 N.D. N.D. -0.6 -0.8
phage K. M5a1 KLEB10 MOI 1 -0.9 N.D. N.D. -0.5 -0.3
phage KLEB17 MOI 1 -0.5 N.D. N.D. -0.2 -1.0
phage KLEB17 MOI 0.1 -0.8 N.D. N.D. -0.5 -0.2
exudates RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x -0.8 N.D. N.D. -0.7 -0.0
nitrogen source Uridine (N) -1.7 N.D. N.D. +0.1 +0.2
stress Nickel (II) chloride 1.5 mM +0.4 N.D. N.D. -0.5 -1.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -0.7 N.D. N.D. -1.0 +0.3
nitrogen source nitrate (N) -0.7 N.D. N.D. -0.8 +0.3
phage KLEB20 MOI 0.1 -0.6 N.D. N.D. -0.7 +0.2
stress Nickel (II) chloride 1.5 mM +0.1 N.D. N.D. +0.2 -1.3
nitrogen source D-Alanine (N) -1.3 N.D. N.D. -0.2 +0.6
mixed community LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL +0.3 N.D. N.D. -0.8 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.6 N.D. N.D. -1.0 +0.4
phage K. M5a1 KLEB23 MOI 1 +0.4 N.D. N.D. -0.7 +0.4
phage K. M5a1 KLEB23 MOI 10 -0.8 N.D. N.D. +0.6 +0.3
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB +0.4 N.D. N.D. -0.7 +0.5
phage KLEB17 MOI 1 -0.6 N.D. N.D. +0.2 +0.8
stress Paraquat dichloride 0.1 mg/ml +0.4 N.D. N.D. -0.7 +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 N.D. N.D. +0.2 +0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 N.D. N.D. -0.2 +1.2
mixed community RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x +1.2 N.D. N.D. -0.2 +0.4
stress Paraquat dichloride 0.1 mg/ml +1.0 N.D. N.D. -0.5 +0.9
stress Sisomicin 0.008 mg/ml +1.0 N.D. N.D. +1.0 -0.3
mixed community LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL +0.5 N.D. N.D. +0.2 +1.0
phage K. M5a1 KLEB26 MOI 0.1 +0.6 N.D. N.D. +0.8 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.3 N.D. N.D. +1.6 +0.0
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x +1.1 N.D. N.D. +0.7 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.6 N.D. N.D. +0.1 +1.3
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