Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS11215 and BWI76_RS11220 overlap by 1 nucleotidesBWI76_RS11220 and BWI76_RS11225 are separated by 48 nucleotidesBWI76_RS11225 and BWI76_RS11230 are separated by 14 nucleotidesBWI76_RS11230 and BWI76_RS11235 are separated by 28 nucleotides BWI76_RS11215: BWI76_RS11215 - lipoprotein ABC transporter ATP-binding protein LolD, at 2,366,761 to 2,367,462 _RS11215 BWI76_RS11220: BWI76_RS11220 - lipoprotein transporter subunit LolE, at 2,367,462 to 2,368,706 _RS11220 BWI76_RS11225: BWI76_RS11225 - N-acetylglucosamine kinase, at 2,368,755 to 2,369,666 _RS11225 BWI76_RS11230: BWI76_RS11230 - NAD-dependent deacylase, at 2,369,681 to 2,370,526 _RS11230 BWI76_RS11235: BWI76_RS11235 - hypothetical protein, at 2,370,555 to 2,370,860 _RS11235
Group Condition BWI76_RS11215 BWI76_RS11220 BWI76_RS11225 BWI76_RS11230 BWI76_RS11235
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days N.D. N.D. N.D. -1.3 -5.5
nitrogen source L-tyrosine (N) N.D. N.D. N.D. +0.3 -4.7
nitrogen source L-Arginine (N) N.D. N.D. N.D. +0.5 -4.6
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x N.D. N.D. N.D. +0.4 -4.1
phage K. M5a1 KLEB10 MOI 1 N.D. N.D. N.D. +1.2 -4.2
phage KLEB15 MOI 1 N.D. N.D. N.D. -0.1 -2.9
stress RCH2_defined_1percent_glucose with Chloride 100 mM N.D. N.D. N.D. -0.1 -2.9
carbon source acetate (C) N.D. N.D. N.D. -2.2 -0.6
ecofab RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL N.D. N.D. N.D. +0.3 -3.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. N.D. -0.7 -2.0
nitrogen source N-Acetyl-D-glucosamine (N) N.D. N.D. N.D. -0.3 -2.3
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x N.D. N.D. N.D. +1.0 -3.6
phage KLEB26 MOI0.1 N.D. N.D. N.D. -0.5 -2.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. N.D. N.D. -0.1 -2.2
carbon source Inosine (C) N.D. N.D. N.D. +0.4 -2.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. +0.3 -2.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X N.D. N.D. N.D. +0.3 -2.1
carbon source 2-Deoxy-D-ribonic lithium salt 10 mM (C) N.D. N.D. N.D. +0.3 -1.8
carbon source 2'-Deoxycytidine 10 mM (C) N.D. N.D. N.D. +0.4 -1.6
micoeukaryotes Acanthamoeba sp., muxed culture-2 N.D. N.D. N.D. +1.1 -1.0
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x N.D. N.D. N.D. +1.2 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. N.D. +2.0 -0.8
phage KLEB1 MOI 10 N.D. N.D. N.D. -0.3 +1.9
stress Fungal_growth_media_mCAFEs_v1 with Thallium(I) acetate 0.05 mg/mL N.D. N.D. N.D. -0.3 +2.1
phage KLEB16 MOI 10 N.D. N.D. N.D. +0.8 +1.2
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM N.D. N.D. N.D. +0.2 +2.0
phage K. M5a1 KLEB26 MOI 0.1 N.D. N.D. N.D. -0.2 +2.4
phage K. M5a1 KLEB23 MOI 1 N.D. N.D. N.D. +1.3 +1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. +2.9 -0.2
phage K. M5a1 KLEB23 MOI 1 N.D. N.D. N.D. +2.0 +1.1
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