Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS11105 and BWI76_RS11110 are separated by 2 nucleotidesBWI76_RS11110 and BWI76_RS11115 overlap by 11 nucleotidesBWI76_RS11115 and BWI76_RS11120 overlap by 4 nucleotidesBWI76_RS11120 and BWI76_RS11125 are separated by 10 nucleotides BWI76_RS11105: BWI76_RS11105 - aminodeoxychorismate lyase, at 2,341,992 to 2,342,801 _RS11105 BWI76_RS11110: BWI76_RS11110 - cell division protein YceG, at 2,342,804 to 2,343,826 _RS11110 BWI76_RS11115: BWI76_RS11115 - dTMP kinase, at 2,343,816 to 2,344,457 _RS11115 BWI76_RS11120: BWI76_RS11120 - DNA polymerase III subunit delta', at 2,344,454 to 2,345,458 _RS11120 BWI76_RS11125: BWI76_RS11125 - metal-dependent hydrolase, at 2,345,469 to 2,346,263 _RS11125
Group Condition BWI76_RS11105 BWI76_RS11110 BWI76_RS11115 BWI76_RS11120 BWI76_RS11125
nitrogen source D-Alanine (N) -6.2 -0.3 N.D. N.D. -0.6
rhizosphere rhizosphere EcoFAB sample 3; outgrowth in LB -0.3 -5.4 N.D. N.D. -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 -1.2 N.D. N.D. -5.0
phage K. M5a1 KLEB10 MOI 10 +0.4 -4.8 N.D. N.D. -1.0
supernatant control Vogels_fungal_media; pH 5.5 -3.8 -1.0 N.D. N.D. -0.6
phage KLEB20 MOI 1 -0.8 +0.9 N.D. N.D. -4.9
mixed community LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL -0.2 -4.3 N.D. N.D. +0.0
micoeukaryotes Acanthamoeba sp., muxed culture-2 -0.4 -1.4 N.D. N.D. -2.6
nitrogen source Thymidine (N) -3.0 -0.7 N.D. N.D. -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 -1.6 N.D. N.D. -1.7
stress Cephalothin 0.16 mg/ml -0.3 -2.8 N.D. N.D. -0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X +0.6 -3.6 N.D. N.D. -0.5
phage KLEB14 MOI 0.1 -0.6 -2.7 N.D. N.D. -0.1
stress copper (II) chloride 3 mM -0.6 -2.1 N.D. N.D. -0.5
phage KLEB1 MOI 10 +0.4 -1.6 N.D. N.D. -1.8
phage KLEB15 MOI 0.1 -2.1 -0.9 N.D. N.D. +0.2
phage KLEB17 MOI 1 -1.6 +1.2 N.D. N.D. -2.2
stress Fungal_growth_media_mCAFEs_v1 with Phosphomycin 0.01 mg/mL -0.7 -2.0 N.D. N.D. +0.6
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml -2.0 -1.0 N.D. N.D. +1.1
mixed community LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL -1.2 -1.2 N.D. N.D. +0.8
stress Cobalt chloride 0.5 mM -1.8 +0.8 N.D. N.D. -0.6
phage K. M5a1 KLEB22 MOI 10 -1.2 -1.2 N.D. N.D. +0.9
mixed community Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL -1.0 -1.4 N.D. N.D. +1.0
carbon source 2-Deoxy-D-ribonic lithium salt 10 mM (C) +0.5 +1.1 N.D. N.D. -2.8
mixed community LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL -0.6 -1.4 N.D. N.D. +1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.6 -2.1 N.D. N.D. +0.9
exudates RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x +0.7 -1.8 N.D. N.D. +0.7
ecofab LB in EcoFAB_10mL +0.2 -1.2 N.D. N.D. +1.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 -1.2 N.D. N.D. +3.5
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 -0.6 N.D. N.D. +4.8
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