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Fitness for 5 genes in
Klebsiella michiganensis M5al
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500 nt
BWI76_RS10490 and BWI76_RS10495 are separated by 125 nucleotides
BWI76_RS10495 and BWI76_RS10500 are separated by 174 nucleotides
BWI76_RS10500 and BWI76_RS10505 are separated by 346 nucleotides
BWI76_RS10505 and BWI76_RS10510 overlap by 23 nucleotides
BWI76_RS10490: BWI76_RS10490 - vanillate O-demethylase oxygenase subunit, at 2,216,391 to 2,217,428
_RS10490
BWI76_RS10495: BWI76_RS10495 - LysR family transcriptional regulator, at 2,217,554 to 2,218,492
_RS10495
BWI76_RS10500: BWI76_RS10500 - flavoprotein monooxygenase, at 2,218,667 to 2,219,821
_RS10500
BWI76_RS10505: BWI76_RS10505 - putative guanine/xanthin permease, at 2,220,168 to 2,221,550
_RS10505
BWI76_RS10510: BWI76_RS10510 - OHCU decarboxylase, at 2,221,528 to 2,222,028
_RS10510
Group
Condition
BWI76
_RS10490
BWI76
_RS10495
BWI76
_RS10500
BWI76
_RS10505
BWI76
_RS10510
nitrogen source
Inosine (N)
-2.7
-5.8
-3.6
-1.8
-5.2
nitrogen source
Adenosine (N)
-1.4
-0.7
-1.1
-1.3
-1.2
nitrogen source
Gly-DL-Asp (N)
-0.0
+0.0
-3.0
-0.4
-0.5
phage
K. M5a1 KLEB26 MOI 0.1
-0.8
+0.0
-0.7
-1.5
-0.1
nitrogen source
L-Arginine (N)
+0.1
-0.0
-1.6
-0.3
-1.0
nitrogen source
Uridine (N)
-0.1
-0.0
-1.8
+0.2
-0.9
phage
KLEB1 MOI 10
-0.3
+0.0
-1.6
+0.2
-1.0
phage
KLEB16 MOI 10
-0.7
-0.3
-1.0
-0.5
-0.1
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x
+0.0
+0.8
-1.8
-0.7
-0.5
nitrogen source
L-Arginine (N)
-1.0
-0.0
-0.4
-1.3
+0.8
phage
K. M5a1 KLEB23 MOI 10
-0.8
-1.0
+0.1
-0.6
+0.7
carbon source
2'-Deoxyinosine 5 mM (C)
-0.1
+0.5
-0.8
+0.3
-1.3
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.9
-0.3
-1.5
-0.5
+0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-0.3
-0.2
-1.3
+1.0
-0.1
nitrogen source
N-Acetyl-D-glucosamine (N)
+0.4
-0.4
-1.3
-0.2
+0.5
phage
K. M5a1 KLEB23 MOI 1
+0.2
-0.4
-0.8
-0.6
+0.8
stress
Cobalt chloride 0.5 mM
+0.1
+0.5
-1.4
+0.0
+0.7
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.3
+1.0
+0.2
-0.2
-1.2
supernatant
Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
-0.0
+0.4
+1.4
-0.0
-1.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+2.0
-0.4
-0.3
-0.1
-0.3
carbon source
Gly-Glu (C)
+0.1
+0.2
+0.5
-0.6
+1.2
stress
Fungal_growth_media_mCAFEs_v1 with Carbenicillin 0.008 mg/mL
+0.9
+0.3
+1.2
-0.2
-0.3
micoeukaryotes
Tetrahymena sp., Mixed culture-1
+0.7
+0.3
+0.9
-0.9
+1.0
rhizosphere
rhizosphere EcoFAB sample 3; outgrowth in LB
+0.4
+0.5
+1.5
+0.1
-0.2
mixed community
RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
+0.4
+0.8
+1.0
+0.7
-0.5
micoeukaryotes
Dictyostellum sp.mixed culture-1
-0.6
-0.4
+2.3
+0.7
+0.6
mixed community
RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
+0.5
+0.4
+1.0
+0.6
+0.2
mixed community
RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
+0.5
+0.1
+1.3
+0.5
+0.6
phage
KLEB17 MOI 0.1
+0.1
+1.0
+1.6
+0.4
+0.1
phage
KLEB1 MOI 0.1
+0.9
+0.6
+1.0
+0.9
+0.4
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