Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS08280 and BWI76_RS08285 are separated by 184 nucleotidesBWI76_RS08285 and BWI76_RS08290 are separated by 32 nucleotidesBWI76_RS08290 and BWI76_RS08295 are separated by 56 nucleotidesBWI76_RS08295 and BWI76_RS08300 are separated by 3 nucleotides BWI76_RS08280: BWI76_RS08280 - acyl-CoA esterase, at 1,751,986 to 1,752,759 _RS08280 BWI76_RS08285: BWI76_RS08285 - replication initiation regulator SeqA, at 1,752,944 to 1,753,492 _RS08285 BWI76_RS08290: BWI76_RS08290 - phosphoglucomutase, alpha-D-glucose phosphate-specific, at 1,753,525 to 1,755,165 _RS08290 BWI76_RS08295: BWI76_RS08295 - hypothetical protein, at 1,755,222 to 1,755,641 _RS08295 BWI76_RS08300: BWI76_RS08300 - DNA-binding response regulator, at 1,755,645 to 1,756,322 _RS08300
Group Condition BWI76_RS08280 BWI76_RS08285 BWI76_RS08290 BWI76_RS08295 BWI76_RS08300
supernatant control Vogels_fungal_media; pH 5.5 -0.9 N.D. N.D. -0.4 N.D.
phage KLEB15 MOI 10 -0.8 N.D. N.D. -0.5 N.D.
phage K. M5a1 KLEB23 MOI 10 -0.4 N.D. N.D. -1.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.8 N.D. N.D. -0.4 N.D.
phage KLEB17 MOI 1 -0.5 N.D. N.D. -0.7 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X -1.2 N.D. N.D. +0.1 N.D.
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM -1.1 N.D. N.D. +0.2 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.0 N.D. N.D. +0.4 N.D.
ecofab RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL -0.8 N.D. N.D. +0.6 N.D.
mixed community RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x +0.5 N.D. N.D. -0.6 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X +0.6 N.D. N.D. -0.6 N.D.
phage KLEB31 MOI 1 +0.6 N.D. N.D. -0.6 N.D.
mixed community RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x +0.7 N.D. N.D. -0.6 N.D.
mixed community RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x +0.7 N.D. N.D. -0.5 N.D.
ecofab RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL -0.7 N.D. N.D. +1.0 N.D.
mixed community LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL +0.8 N.D. N.D. -0.6 N.D.
phage K. M5a1 KLEB26 MOI 0.1 -0.5 N.D. N.D. +0.8 N.D.
phage KLEB17 MOI 0.1 +0.9 N.D. N.D. -0.3 N.D.
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x +1.0 N.D. N.D. -0.3 N.D.
rhizosphere rhizosphere sample 1; outgrowth in LB +1.2 N.D. N.D. -0.5 N.D.
mixed community RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x +1.2 N.D. N.D. +0.0 N.D.
nitrogen source L-Asparagine (N) +1.3 N.D. N.D. -0.1 N.D.
nitrogen source L-Lysine (N) +0.8 N.D. N.D. +0.4 N.D.
nitrogen source Glucuronamide (N) +1.1 N.D. N.D. +0.2 N.D.
phage KLEB1 MOI 10 +1.0 N.D. N.D. +0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +1.2 N.D. N.D. +0.2 N.D.
stress Sisomicin 0.008 mg/ml +1.5 N.D. N.D. +0.2 N.D.
stress Cobalt chloride 0.5 mM +1.0 N.D. N.D. +0.6 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 N.D. N.D. +1.7 N.D.
stress Sisomicin 0.008 mg/ml +2.3 N.D. N.D. +0.7 N.D.
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