Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS07255 and BWI76_RS07260 are separated by 17 nucleotidesBWI76_RS07260 and BWI76_RS07265 are separated by 27 nucleotidesBWI76_RS07265 and BWI76_RS07270 overlap by 8 nucleotidesBWI76_RS07270 and BWI76_RS07275 overlap by 4 nucleotides BWI76_RS07255: BWI76_RS07255 - hypothetical protein, at 1,527,258 to 1,527,740 _RS07255 BWI76_RS07260: BWI76_RS07260 - branched chain amino acid ABC transporter substrate-binding protein, at 1,527,758 to 1,528,915 _RS07260 BWI76_RS07265: BWI76_RS07265 - ABC transporter ATP-binding protein, at 1,528,943 to 1,529,662 _RS07265 BWI76_RS07270: BWI76_RS07270 - ABC transporter ATP-binding protein, at 1,529,655 to 1,530,422 _RS07270 BWI76_RS07275: BWI76_RS07275 - ABC transporter, at 1,530,419 to 1,531,525 _RS07275
Group Condition BWI76_RS07255 BWI76_RS07260 BWI76_RS07265 BWI76_RS07270 BWI76_RS07275
phage K. M5a1 KLEB26 MOI 0.1 -0.5 +0.5 -1.8 -0.0 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.2 -1.0 -1.1 +0.1 +0.0
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X -0.8 -0.7 -0.6 +0.3 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.7 -0.6 -0.0 +0.7 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.3 -0.3 -0.3 +0.1 +0.2
ecofab RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL -1.3 -0.1 +0.5 +0.2 -0.7
nitrogen source Casamino Acids (N) -0.8 -0.7 +0.7 -0.1 -0.0
mixed community LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL +0.6 -0.5 -0.8 -0.4 +0.2
carbon source 2-Deoxy-D-ribonic lithium salt 10 mM (C) +0.2 -0.6 -0.3 +0.6 -0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -1.3 +0.2 +0.4 +0.2 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.8 -0.2 +0.8 +0.2 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.4 +0.5 +0.8 -0.4 +0.3
ecofab LB in EcoFAB_3.5mL +0.2 +1.0 -0.7 -0.2 -0.1
rhizosphere rhizosphere sample 1; outgrowth in LB -0.8 -0.3 +0.1 +0.3 +1.1
nitrogen source Cytosine (N) -1.3 +0.2 +0.9 +0.4 +0.3
mixed community RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x +1.1 -0.8 +0.4 -0.2 +0.1
carbon source 2-Deoxy-D-ribonic lithium salt 10 mM (C) +0.5 +0.2 +0.6 +0.5 -0.7
ecofab RCH2_defined_Glucose_noNitrogen in EcoFAB_3.5mL +2.0 -0.4 +0.0 -0.3 -0.1
nitrogen source D-Alanine (N) +0.7 -0.3 +0.9 -0.4 +0.4
mixed community Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL +0.6 +0.6 +0.9 -0.7 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.9 -0.3 +0.2 -0.1 -0.2
nitrogen source L-Phenylalanine (N) +0.6 -0.4 +0.8 +0.4 +0.2
mixed community RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x +1.3 +0.0 -0.4 +0.6 +0.1
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x +1.1 -0.6 +0.7 +0.3 +0.2
mixed community RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x +1.6 +0.1 +0.4 +0.2 -0.1
phage K. M5a1 KLEB10 MOI 1 +0.8 +0.2 +0.6 +0.3 +0.3
phage KLEB17 MOI 1 +0.8 +1.1 -0.1 +0.5 -0.0
phage KLEB17 MOI 1 +0.0 +0.9 +0.8 +0.3 +0.3
exudates RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x +0.1 +0.5 +1.4 +0.4 +0.3
stress Sisomicin 0.008 mg/ml +2.7 +0.8 +0.3 +0.3 +0.0
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