Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS05430 and BWI76_RS05435 are separated by 57 nucleotidesBWI76_RS05435 and BWI76_RS05440 are separated by 71 nucleotidesBWI76_RS05440 and BWI76_RS05445 are separated by 32 nucleotidesBWI76_RS05445 and BWI76_RS05450 overlap by 4 nucleotides BWI76_RS05430: BWI76_RS05430 - UbiE/COQ5 family methyltransferase, at 1,140,188 to 1,140,958 _RS05430 BWI76_RS05435: BWI76_RS05435 - murein transglycosylase D, at 1,141,016 to 1,142,383 _RS05435 BWI76_RS05440: BWI76_RS05440 - hydroxyacylglutathione hydrolase, at 1,142,455 to 1,143,210 _RS05440 BWI76_RS05445: BWI76_RS05445 - type 11 methyltransferase, at 1,143,243 to 1,143,965 _RS05445 BWI76_RS05450: BWI76_RS05450 - ribonuclease HI, at 1,143,962 to 1,144,429 _RS05450
Group Condition BWI76_RS05430 BWI76_RS05435 BWI76_RS05440 BWI76_RS05445 BWI76_RS05450
phage KLEB1 MOI 10 -0.7 -0.6 -0.4 +0.0 -3.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 -0.2 +0.1 -0.3 -3.3
nitrogen source L-Arginine (N) -0.4 +0.3 -0.2 +0.2 -4.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X -0.6 -0.0 -0.1 +0.3 -3.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.6 -0.7 +0.0 -0.3 -2.3
phage KLEB1 MOI 10 -0.9 -0.4 -0.3 +0.1 -2.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 -0.3 -0.2 +0.2 -3.3
phage KLEB16 MOI 10 +0.5 +0.1 -0.6 +0.4 -3.7
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x +0.1 -0.0 +0.1 +0.3 -3.7
phage KLEB16 MOI 0.1 -0.1 +0.0 +0.1 +0.5 -3.6
phage KLEB14 MOI 0.1 +0.3 +0.1 +0.0 -0.5 -3.0
phage KLEB16 MOI 0.1 +0.3 +0.2 -0.0 +0.3 -3.6
supernatant control:fungal media R2A with Vogels_fungal_media 0.2X +0.4 +0.1 -0.6 +0.1 -2.8
micoeukaryotes Acanthamoeba sp., muxed culture-2 +0.4 -0.3 +0.1 +0.5 -3.4
nitrogen source Glucuronamide (N) +0.8 +0.0 +0.0 +0.4 -3.9
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x +1.0 +0.0 -0.3 -1.0 -2.2
stress Sisomicin 0.008 mg/ml +0.1 -1.2 -0.6 +1.0 -1.8
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 83.25 mM +0.7 +0.1 -0.1 -0.1 -3.0
mixed community RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x +0.5 +0.4 -0.5 -0.4 -2.2
nitrogen source L-Arginine (N) -0.4 +0.6 +0.4 +0.2 -3.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 +0.4 +0.1 -0.0 -3.1
phage KLEB16 MOI 1 +0.2 +0.5 +0.0 +0.5 -2.9
stress perchlorate 100 mM +0.5 +0.4 +0.0 +0.3 -2.8
mixed community RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x +0.7 -0.1 -0.1 +1.0 -2.2
mixed community RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x +0.7 -0.1 -0.6 +1.0 -1.5
phage K. M5a1 KLEB22 MOI 10 -0.2 -0.3 +0.3 -0.4 +3.2
phage K. M5a1 KLEB23 MOI 10 +0.0 -0.4 +0.3 -0.0 +3.3
phage K. M5a1 KLEB23 MOI 10 +0.1 -0.3 +0.1 +0.1 +3.4
stress Cobalt chloride 0.5 mM +0.0 -0.4 +0.3 -0.4 +4.2
stress Cobalt chloride 0.5 mM +0.5 -0.4 +0.3 +0.2 +4.4
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