Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS04875 and BWI76_RS04880 are separated by 119 nucleotidesBWI76_RS04880 and BWI76_RS04885 are separated by 14 nucleotidesBWI76_RS04885 and BWI76_RS04890 are separated by 207 nucleotidesBWI76_RS04890 and BWI76_RS04895 are separated by 162 nucleotides BWI76_RS04875: BWI76_RS04875 - transcriptional regulator PdhR, at 1,019,107 to 1,019,886 _RS04875 BWI76_RS04880: BWI76_RS04880 - pyruvate dehydrogenase (acetyl-transferring), homodimeric type, at 1,020,006 to 1,022,669 _RS04880 BWI76_RS04885: BWI76_RS04885 - pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase, at 1,022,684 to 1,024,573 _RS04885 BWI76_RS04890: BWI76_RS04890 - dihydrolipoyl dehydrogenase, at 1,024,781 to 1,026,205 _RS04890 BWI76_RS04895: BWI76_RS04895 - hypothetical protein, at 1,026,368 to 1,026,652 _RS04895
Group Condition BWI76_RS04875 BWI76_RS04880 BWI76_RS04885 BWI76_RS04890 BWI76_RS04895
agar plate interaction Taped volatile agar plate with Trichoderma atroviridae IMI -0.9 N.D. N.D. N.D. -1.3
nitrogen source Gly-DL-Asp (N) -0.3 N.D. N.D. N.D. -1.9
nitrogen source L-Cysteine (N) -1.1 N.D. N.D. N.D. -0.8
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM -0.8 N.D. N.D. N.D. -0.9
supernatant control Vogels_fungal_media; pH 5.5 -1.0 N.D. N.D. N.D. -0.7
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X -1.1 N.D. N.D. N.D. -0.5
stress nitrite 40 mM -0.8 N.D. N.D. N.D. -0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.1 N.D. N.D. N.D. -0.3
phage KLEB31 MOI 1 -0.6 N.D. N.D. N.D. -0.9
mixed community RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x +0.4 N.D. N.D. N.D. -1.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.7 N.D. N.D. N.D. +0.4
nitrogen source D-Alanine (N) -1.3 N.D. N.D. N.D. +0.2
carbon source D-Serine (C) -1.2 N.D. N.D. N.D. +0.3
phage KLEB17 MOI 1 -1.8 N.D. N.D. N.D. +1.0
phage KLEB20 MOI 1 -1.4 N.D. N.D. N.D. +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.8 N.D. N.D. N.D. +1.1
supernatant RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5 x; and 2,2'-Dipyridyl 50 uM -0.4 N.D. N.D. N.D. +1.0
phage KLEB15 MOI 10 -0.5 N.D. N.D. N.D. +1.4
mixed community Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL +0.6 N.D. N.D. N.D. +0.7
ecofab LB in EcoFAB_3.5mL with sand +0.2 N.D. N.D. N.D. +1.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +0.4 N.D. N.D. N.D. +1.1
mixed community LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL +0.6 N.D. N.D. N.D. +0.9
mixed community Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL +0.4 N.D. N.D. N.D. +1.2
mixed community LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL +0.1 N.D. N.D. N.D. +1.5
mixed community RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x +0.8 N.D. N.D. N.D. +0.8
carbon source D-Lactate (C) +1.4 N.D. N.D. N.D. +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.4 N.D. N.D. N.D. +2.6
stress Fungal_growth_media_mCAFEs_v1 with Vancomycin 0.01 mg/mL +0.9 N.D. N.D. N.D. +1.4
stress Sisomicin 0.008 mg/ml +5.0 N.D. N.D. N.D. -0.1
stress Sisomicin 0.008 mg/ml +7.8 N.D. N.D. N.D. +0.0
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