Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS04695 and BWI76_RS04700 are separated by 2 nucleotidesBWI76_RS04700 and BWI76_RS04705 are separated by 180 nucleotidesBWI76_RS04705 and BWI76_RS04710 are separated by 444 nucleotidesBWI76_RS04710 and BWI76_RS04715 overlap by 2 nucleotides BWI76_RS04695: BWI76_RS04695 - acetolactate synthase 3 large subunit, at 979,896 to 981,620 _RS04695 BWI76_RS04700: BWI76_RS04700 - acetolactate synthase small subunit, at 981,623 to 982,114 _RS04700 BWI76_RS04705: BWI76_RS04705 - DNA-binding transcriptional regulator FruR, at 982,295 to 983,299 _RS04705 BWI76_RS04710: BWI76_RS04710 - hypothetical protein, at 983,744 to 983,926 _RS04710 BWI76_RS04715: BWI76_RS04715 - division/cell wall cluster transcriptional repressor MraZ, at 983,925 to 984,383 _RS04715
Group Condition BWI76_RS04695 BWI76_RS04700 BWI76_RS04705 BWI76_RS04710 BWI76_RS04715
rhizosphere rhizosphere EcoFAB sample 3; outgrowth in LB -0.0 +0.1 -6.1 -1.4 -4.8
carbon source succinate (C) +0.1 +0.1 -5.2 -0.7 -2.9
lb dilute Dilute LB (1/10) -0.1 +0.1 -3.8 -0.1 -4.1
lb dilute Dilute LB (1/10) -0.0 -0.1 -3.4 -0.6 -3.8
stress Sisomicin 0.008 mg/ml -0.0 -0.1 -3.2 -0.5 -3.9
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM +0.0 -0.2 -2.1 -0.6 -4.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 -0.3 -2.9 +0.5 -4.2
carbon source pyruvate (C) -0.0 +0.1 -4.5 -0.2 -2.2
carbon source succinate (C) +0.0 -0.5 -5.1 +0.1 -1.0
rhizosphere rhizosphere sample 1; outgrowth in LB +0.2 -0.1 -2.5 -0.2 -4.0
carbon source L-Glutamine (C) -0.1 +0.4 -4.5 -0.3 -1.7
carbon source D-Alanine (C) -0.0 +0.4 -5.0 -0.4 -1.1
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM -0.1 -0.1 -2.9 -0.5 -2.5
mixed community RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x -0.1 +0.7 -3.2 -0.1 -3.4
carbon source L-Lactate (C) -0.2 +0.4 -4.4 -0.6 -1.2
carbon source D-Alanine (C) +0.1 +0.3 -4.7 -1.0 -0.7
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM -0.2 +0.0 -2.6 -0.1 -3.1
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM -0.1 -0.1 -2.5 +0.1 -3.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 -0.4 -3.5 -0.2 -1.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 -0.3 -2.7 +1.0 -3.6
carbon source D,L-Malic Acid (C) -0.1 +0.6 -4.1 -0.8 -1.2
agar plate interaction Taped volatile agar plate with Trichoderma atroviridae IMI +0.2 -0.4 -2.1 +1.0 -4.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 +0.6 -2.3 +0.1 -4.2
supernatant Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x +0.1 +0.2 -2.3 -0.3 -3.1
carbon source L-Lactate (C) -0.2 +0.1 -4.7 -0.4 +0.4
stress Cephalothin 0.16 mg/ml +0.1 +0.2 +0.6 -1.2 -4.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 +0.6 -2.0 -1.1 -2.2
carbon source L-Sorbose (C) +0.1 -0.1 -4.4 -0.0 +1.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 +0.9 -2.4 +0.7 -2.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 +0.3 -3.0 +2.9 -1.5
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