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Fitness for 5 genes in
Klebsiella michiganensis M5al
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500 nt
BWI76_RS04300 and BWI76_RS04305 are separated by 87 nucleotides
BWI76_RS04305 and BWI76_RS04310 are separated by 327 nucleotides
BWI76_RS04310 and BWI76_RS04315 are separated by 505 nucleotides
BWI76_RS04315 and BWI76_RS04320 are separated by 53 nucleotides
BWI76_RS04300: BWI76_RS04300 - molecular chaperone DnaK, at 885,079 to 886,995
_RS04300
BWI76_RS04305: BWI76_RS04305 - molecular chaperone DnaJ, at 887,083 to 888,219
_RS04305
BWI76_RS04310: BWI76_RS04310 - hypothetical protein, at 888,547 to 888,726
_RS04310
BWI76_RS04315: BWI76_RS04315 - Na+/H+ antiporter NhaA, at 889,232 to 890,407
_RS04315
BWI76_RS04320: BWI76_RS04320 - transcriptional activator NhaR, at 890,461 to 891,360
_RS04320
Group
Condition
BWI76
_RS04300
BWI76
_RS04305
BWI76
_RS04310
BWI76
_RS04315
BWI76
_RS04320
stress
RCH2_defined_1percent_glucose with Chloride 600 mM
N.D.
-5.0
+0.0
-4.4
+0.3
pH
LB, pH10
N.D.
-3.1
+0.0
-3.4
-2.6
stress
RCH2_defined_1percent_glucose with Chloride 600 mM
N.D.
-5.1
+0.0
-3.4
-0.3
stress
RCH2_defined_1percent_glucose with Chloride 600 mM and Betaine 100 uM
N.D.
-5.1
-0.4
-1.8
+0.0
nitrogen source
L-tyrosine (N)
N.D.
-1.2
-0.2
-3.0
-1.3
stress
RCH2_defined_1percent_glucose with Chloride 400 mM
N.D.
-3.6
-0.2
-1.7
-0.1
nitrogen source
L-Arginine (N)
N.D.
-5.0
-0.2
+0.1
-0.5
nitrogen source
nitrate (N)
N.D.
-5.1
+0.1
-0.3
-0.2
carbon source
Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM
N.D.
-5.1
-0.1
+0.1
-0.3
supernatant control
Vogels_fungal_media; pH 5.5
N.D.
-4.7
+0.1
-0.4
-0.3
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM
N.D.
-5.3
-0.3
+0.2
+0.1
nitrogen source
L-Tryptophan (N)
N.D.
-5.1
-0.2
+0.2
-0.2
supernatant
RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
N.D.
-4.8
-0.2
+0.1
-0.4
nitrogen fixation
D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days
N.D.
-4.5
-0.1
+0.2
-0.6
pH
LB, pH9
N.D.
-3.1
+0.3
-2.0
-0.1
no stress control
RCH2_defined_1percent_glucose; no stress
N.D.
-5.1
-0.2
+0.3
+0.1
supernatant
Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
N.D.
-4.1
+0.4
+0.4
-1.5
phage
KLEB17 MOI 1
N.D.
-3.7
-0.3
+0.5
-1.1
mixed community
Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
N.D.
-4.7
-0.3
+0.5
-0.1
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X
N.D.
-4.8
+0.5
-0.0
-0.3
nitrogen source
Thymine (N)
N.D.
-5.0
+0.1
+0.1
+0.1
nitrogen source
Thymidine (N)
N.D.
-5.1
-0.0
+0.2
+0.4
stress
Fungal_growth_media_mCAFEs_v1 with Zinc 2 mM
N.D.
-4.6
-0.1
+0.5
+0.2
supernatant
Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
N.D.
-4.7
+0.3
+0.6
-0.2
nitrogen source
Gly-DL-Asp (N)
N.D.
-4.7
+0.5
+0.1
+0.2
mixed community
LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
N.D.
-4.5
-0.2
+0.8
-0.0
stress
nitrite 20 mM
N.D.
-4.7
+0.0
+0.4
+0.3
supernatant
Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml
N.D.
-4.7
+0.5
+0.6
-0.3
nitrogen source
Cytosine (N)
N.D.
-4.8
+0.1
+0.2
+0.7
carbon source
2-Deoxyadenosine 5-monophosphate 5 mM (C)
N.D.
-4.4
+0.4
+0.7
+0.4
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