Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS02915 and BWI76_RS02920 are separated by 18 nucleotidesBWI76_RS02920 and BWI76_RS02925 are separated by 25 nucleotidesBWI76_RS02925 and BWI76_RS02930 are separated by 17 nucleotidesBWI76_RS02930 and BWI76_RS02935 are separated by 257 nucleotides BWI76_RS02915: BWI76_RS02915 - Hcp family T6SS protein Cts2H, at 595,729 to 596,214 _RS02915 BWI76_RS02920: BWI76_RS02920 - addiction module toxin RelE, at 596,233 to 596,379 _RS02920 BWI76_RS02925: BWI76_RS02925 - type IV secretion protein Rhs, at 596,405 to 598,123 _RS02925 BWI76_RS02930: BWI76_RS02930 - anaerobic ribonucleoside-triphosphate reductase activating protein, at 598,141 to 598,605 _RS02930 BWI76_RS02935: BWI76_RS02935 - anaerobic ribonucleoside triphosphate reductase, at 598,863 to 601,001 _RS02935
Group Condition BWI76_RS02915 BWI76_RS02920 BWI76_RS02925 BWI76_RS02930 BWI76_RS02935
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days +0.1 +0.1 +0.0 -4.7 -4.7
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 3 days -0.0 -0.3 -0.1 -2.0 -2.0
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days -0.1 -0.4 -0.0 -1.7 -1.8
mixed community RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF -0.3 -0.0 +0.0 -0.9 -0.8
mixed community LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL -0.4 -1.0 -0.1 -0.4 -0.0
phage KLEB20 MOI 0.1 -0.1 -1.2 -0.1 +0.1 -0.4
nitrogen source D-Alanine (N) -0.6 -0.9 +0.4 -0.5 +0.2
stress Paraquat dichloride 0.1 mg/ml -0.7 -0.2 -0.6 -0.1 +0.3
mixed community RCH2_defined_Glucose_noNitrogen in Eppendorf tube with sand, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF +0.1 -0.1 +0.4 -1.0 -0.6
phage KLEB1 MOI 0.1 -0.2 +0.4 -0.5 -0.3 -0.5
stress Fungal_growth_media_mCAFEs_v1 with Thallium(I) acetate 0.05 mg/mL -0.4 +0.5 +0.1 -0.4 -0.5
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml +0.3 -0.4 +0.5 -0.1 -0.7
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x -0.4 -0.6 -0.2 -0.1 +0.8
carbon source D-Salicin (C) +0.4 -0.7 -0.4 +0.4 -0.1
mixed community Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL +0.3 +0.8 -0.1 -0.6 -0.7
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.4 +0.2 +0.5 -0.9 -0.4
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml +0.1 +0.6 +0.3 -0.6 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 +1.5 -0.1 -0.6 -0.1
stress Fungal_growth_media_mCAFEs_v1 with Vancomycin 0.01 mg/mL +0.3 +0.7 +0.3 -0.3 -0.4
stress Fungal_growth_media_mCAFEs_v1 with Vancomycin 0.01 mg/mL -0.1 +0.6 +0.5 +0.1 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 +0.1 -0.3 +1.1 -0.1
mixed community RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x -0.4 +0.5 +0.7 +0.2 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 +0.8 -0.2 -0.4 +0.4
mixed community RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x +0.3 +0.8 +0.4 -0.2 -0.2
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 6 x +0.2 +0.4 +0.7 +0.2 -0.3
supernatant RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x +0.1 +0.8 -0.2 +0.6 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 +0.1 +0.2 +1.4 -0.1
nitrogen source L-tyrosine (N) +0.3 +0.5 +0.5 +0.6 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.3 +0.0 -0.4 +1.9 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.1 -0.0 +0.2 +2.2 -0.2
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