Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS01525 and BWI76_RS01530 are separated by 318 nucleotidesBWI76_RS01530 and BWI76_RS01535 overlap by 4 nucleotidesBWI76_RS01535 and BWI76_RS01540 are separated by 1 nucleotidesBWI76_RS01540 and BWI76_RS01545 overlap by 17 nucleotides BWI76_RS01525: BWI76_RS01525 - sensor domain-containing diguanylate cyclase, at 310,703 to 312,235 _RS01525 BWI76_RS01530: BWI76_RS01530 - 2-iminoacetate synthase ThiH, at 312,554 to 313,687 _RS01530 BWI76_RS01535: BWI76_RS01535 - thiazole synthase, at 313,684 to 314,454 _RS01535 BWI76_RS01540: BWI76_RS01540 - thiamine biosynthesis protein ThiS, at 314,456 to 314,656 _RS01540 BWI76_RS01545: BWI76_RS01545 - thiamine biosynthesis protein ThiS, at 314,640 to 315,395 _RS01545
Group Condition BWI76_RS01525 BWI76_RS01530 BWI76_RS01535 BWI76_RS01540 BWI76_RS01545
carbon source Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM +0.1 -2.3 -3.1 -3.0 -1.8
supernatant control Vogels_fungal_media; pH 5.5 +0.2 -2.6 -1.1 -4.4 -1.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +0.2 -1.7 -1.8 -3.0 -2.5
carbon source Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM +0.0 -2.2 -1.9 -1.6 -2.9
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X +0.1 -2.2 -1.7 -1.4 -2.6
stress Fungal_growth_media_mCAFEs_v1 with Zinc 2 mM -0.1 -2.3 -1.2 -1.2 -2.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X +0.2 -1.9 -1.9 -1.3 -2.2
supernatant RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x +0.3 -1.4 -1.2 -1.5 -3.0
mixed community Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL +0.1 -1.3 -1.0 -1.7 -2.2
stress Fungal_growth_media_mCAFEs_v1 with Phosphomycin 0.01 mg/mL +0.2 -1.8 -1.0 -2.2 -1.0
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.33x; and D-Glucose 74 mM -0.1 -1.4 -1.3 -1.4 -1.6
stress Fungal_growth_media_mCAFEs_v1 with Thallium(I) acetate 0.05 mg/mL +0.1 -1.1 -1.4 -1.3 -1.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X -0.0 -1.2 -1.8 -0.4 -1.8
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x -0.0 -0.4 -1.0 -1.1 -2.9
mixed community Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL +0.3 -1.1 -1.5 -1.6 -1.5
mixed community Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL -0.1 -1.2 -0.8 -1.8 -1.3
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 83.25 mM +0.2 -1.2 -1.0 -2.1 -0.9
stress Fungal_growth_media_mCAFEs_v1 with nitrate 20 mM +0.1 -1.7 -1.3 -1.4 -0.7
stress Fungal_growth_media_mCAFEs_v1 with Carbenicillin 0.008 mg/mL +0.1 -0.7 -1.5 -1.8 -1.2
stress Fungal_growth_media_mCAFEs_v1 with Vancomycin 0.01 mg/mL -0.3 -1.5 -1.2 -1.3 -0.7
stress Fungal_growth_media_mCAFEs_v1 with Zinc 2 mM +0.2 -1.9 -0.8 -1.5 -1.0
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x; and D-Glucose 99.9 mM +0.1 -0.8 -1.3 -1.5 -1.3
carbon source RCH2_defined_glucose with MS_media 0.5x +0.0 -1.2 -0.7 -1.4 -1.1
exudates RCH2_defined_glucose with Brachypodium distachyon 27-day exudates 1x +0.0 -1.4 -1.2 -1.2 -0.6
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 83.25 mM -0.1 -1.1 -0.9 -1.2 -1.0
supernatant RCH2_defined_glucose with Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x +0.2 -1.0 -0.9 -1.6 -0.9
stress RCH2_defined_1percent_glucose with Chloride 100 mM +0.1 -0.0 -0.4 -2.4 -1.3
mixed community LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL -0.1 -0.2 +0.5 -2.6 +1.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 -0.1 -0.3 +3.1 +1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.6 -0.3 -0.1 +4.5 +0.9
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