Fitness for 5 genes in Klebsiella michiganensis M5al

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500 ntBWI76_RS01000 and BWI76_RS01005 are separated by 194 nucleotidesBWI76_RS01005 and BWI76_RS01010 are separated by 24 nucleotidesBWI76_RS01010 and BWI76_RS01015 overlap by 1 nucleotidesBWI76_RS01015 and BWI76_RS01020 are separated by 102 nucleotides BWI76_RS01000: BWI76_RS01000 - peptidyl-prolyl cis-trans isomerase, at 204,714 to 204,995 _RS01000 BWI76_RS01005: BWI76_RS01005 - CarD family transcriptional regulator, at 205,190 to 205,894 _RS01005 BWI76_RS01010: BWI76_RS01010 - uridine phosphorylase, at 205,919 to 206,668 _RS01010 BWI76_RS01015: BWI76_RS01015 - nicotinamide riboside transporter PnuC, at 206,668 to 207,390 _RS01015 BWI76_RS01020: BWI76_RS01020 - DNA helicase Rep, at 207,493 to 209,511 _RS01020
Group Condition BWI76_RS01000 BWI76_RS01005 BWI76_RS01010 BWI76_RS01015 BWI76_RS01020
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -1.7 -0.2 +0.2 -0.4 N.D.
supernatant Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x -1.7 -0.0 -0.2 -0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.9 -1.0 +0.1 -0.3 N.D.
nitrogen source Uridine (N) -1.1 -0.3 +0.3 -0.6 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.5 -0.6 -0.0 +0.7 N.D.
rhizosphere rhizosphere EcoFAB sample 3; outgrowth in LB -1.5 +0.3 -0.9 +0.7 N.D.
nitrogen source D-Alanine (N) +0.0 -0.6 -0.9 +0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -1.2 +0.4 -0.1 -0.3 N.D.
nitrogen source D-Serine (N) -1.0 -0.4 +0.2 +0.3 N.D.
nitrogen source L-Arginine (N) -0.6 +0.4 -0.7 +0.2 N.D.
mixed community RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x +0.4 -0.9 +0.5 -0.5 N.D.
phage KLEB1 MOI 10 +0.6 -0.0 +0.4 -1.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.7 -0.4 +1.2 -0.2 N.D.
mixed community Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL -0.6 -0.4 +0.6 +0.4 N.D.
mixed community RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x +0.4 -0.6 +0.6 -0.2 N.D.
nitrogen source Casamino Acids (N) -0.8 +0.8 +0.3 -0.0 N.D.
carbon source 2'-Deoxycytidine 10 mM (C) -0.7 +0.4 +0.2 +0.5 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.2 -0.2 -0.5 +0.1 N.D.
phage KLEB1 MOI 10 +1.0 -0.3 +0.3 -0.2 N.D.
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 6 x +1.0 -0.1 +0.4 -0.4 N.D.
mixed community LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL +0.8 +0.2 +0.4 -0.5 N.D.
nitrogen source L-Phenylalanine (N) +0.5 +0.9 -0.5 +0.1 N.D.
mixed community RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x +1.1 -0.2 +0.4 -0.1 N.D.
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml -0.2 -0.2 +1.0 +0.7 N.D.
carbon source Gly-Glu (C) +0.3 -0.4 +1.1 +0.3 N.D.
mixed community Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL +0.6 +0.1 +0.8 +0.4 N.D.
mixed community RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x +0.4 +0.4 +0.6 +0.4 N.D.
rhizosphere rhizosphere sample 1; outgrowth in LB -0.4 +1.2 +0.7 +0.4 N.D.
supernatant Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x +1.0 +0.2 +0.4 +0.5 N.D.
mixed community Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL +1.0 +0.3 +1.0 +0.9 N.D.
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