Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS36205 and BPHYT_RS36210 overlap by 4 nucleotidesBPHYT_RS36210 and BPHYT_RS36215 are separated by 127 nucleotidesBPHYT_RS36215 and BPHYT_RS36220 are separated by 51 nucleotidesBPHYT_RS36220 and BPHYT_RS36225 overlap by 23 nucleotides BPHYT_RS36205: BPHYT_RS36205 - integrase, at 56,148 to 58,031 _RS36205 BPHYT_RS36210: BPHYT_RS36210 - hypothetical protein, at 58,028 to 58,390 _RS36210 BPHYT_RS36215: BPHYT_RS36215 - hypothetical protein, at 58,518 to 59,036 _RS36215 BPHYT_RS36220: BPHYT_RS36220 - hypothetical protein, at 59,088 to 59,288 _RS36220 BPHYT_RS36225: BPHYT_RS36225 - hypothetical protein, at 59,266 to 59,538 _RS36225
Group Condition BPHYT_RS36205 BPHYT_RS36210 BPHYT_RS36215 BPHYT_RS36220 BPHYT_RS36225
phage P. phyto PHYTO2 MOI 1 N.D. N.D. -0.7 -4.5 -0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. +0.0 -4.9 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=15_days N.D. N.D. +0.1 -4.0 -0.1
phage 36hr PHYTO6 MOI 1 N.D. N.D. +0.2 -3.9 -0.1
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. N.D. -0.1 -3.4 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X N.D. N.D. -1.0 -2.3 -0.1
carbon source 2-Deoxy-D-Ribose (C) N.D. N.D. -0.9 -2.1 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. N.D. -0.9 -2.0 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. -1.6 -1.2 -0.2
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days N.D. N.D. +0.7 -3.3 -0.2
carbon source Gly-Glu (C) N.D. N.D. -0.3 -1.8 -0.5
carbon source Phenylacetic 5 mM (C) N.D. N.D. -0.4 -2.2 +0.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. N.D. -0.5 -2.0 -0.1
phage 24hr PHYTO4 MOI 10 N.D. N.D. +0.3 -2.2 -0.6
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. N.D. -0.1 -2.9 +0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. N.D. +0.3 -2.6 -0.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days N.D. N.D. -0.3 -2.1 -0.0
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.6 -1.9 -0.6
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. -0.2 -1.7 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. -1.5 +0.9 -0.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.7 -1.6 +0.4
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) N.D. N.D. -0.9 +1.4 -0.5
phage 24hr PHYTO6 MOI 10 N.D. N.D. +1.0 +0.3 -1.0
phage 36hr PHYTO10 MOI 10 N.D. N.D. -0.2 +1.8 -0.6
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. -1.0 +1.4 +0.6
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) N.D. N.D. -0.3 +2.0 -0.6
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) N.D. N.D. +0.1 +2.3 -0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -0.9 +2.8 -0.3
nitrogen source L-Lysine (N) N.D. N.D. +0.7 +1.8 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.2 +4.0 -1.0
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