Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS35645 and BPHYT_RS35650 are separated by 120 nucleotidesBPHYT_RS35650 and BPHYT_RS35655 are separated by 230 nucleotidesBPHYT_RS35655 and BPHYT_RS35660 are separated by 62 nucleotidesBPHYT_RS35660 and BPHYT_RS35665 overlap by 4 nucleotides BPHYT_RS35645: BPHYT_RS35645 - porin, at 3,589,838 to 3,590,998 _RS35645 BPHYT_RS35650: BPHYT_RS35650 - DSBA oxidoreductase, at 3,591,119 to 3,591,784 _RS35650 BPHYT_RS35655: BPHYT_RS35655 - hypothetical protein, at 3,592,015 to 3,592,308 _RS35655 BPHYT_RS35660: BPHYT_RS35660 - membrane protein, at 3,592,371 to 3,593,222 _RS35660 BPHYT_RS35665: BPHYT_RS35665 - ABC transporter permease, at 3,593,219 to 3,594,109 _RS35665
Group Condition BPHYT_RS35645 BPHYT_RS35650 BPHYT_RS35655 BPHYT_RS35660 BPHYT_RS35665
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 +0.1 -1.1 -0.5 -0.8
carbon source D-Lactate (C) -0.6 -1.0 -0.4 -0.3 -0.4
phage P. phyto PHYTO2 MOI 1 -0.4 -0.9 -0.7 -0.2 -0.3
carbon source Sebacic (C) -0.0 -0.2 -1.8 -0.2 -0.0
carbon source D-Galactose (C) -0.6 -0.3 -0.1 -0.3 -1.0
phage 36hr PHYTO2 MOI 1 +0.1 -0.6 -1.2 -0.0 -0.6
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.3 -1.3 -1.2 +0.2 +0.4
carbon source 2-Deoxy-D-ribonic lithium salt (C) -0.4 -0.2 -1.7 +0.0 +0.6
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -1.3 -0.8 +0.2 +0.1
phage 24hr PHYTO7 MOI 10 -0.4 -0.9 -0.4 +0.4 -0.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.0 -0.3 -1.6 -0.1 +0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.2 +0.7 -1.4 -0.5 -0.2
motility outer cut, LB soft agar motility assay +0.3 -1.0 -0.7 -0.3 +0.6
phage 36hr PHYTO4 MOI 1 +0.1 -0.1 -1.6 +0.1 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -0.9 -1.1 +0.0 +1.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.2 -0.4 +1.1 -0.8 -0.5
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.5 -0.7 -1.0 +0.3 +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 -1.0 +0.1 -0.7 +0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 +0.4 -1.3 +0.6 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.4 -0.4 +0.7 +0.5 -0.3
carbon source D-Galacturonic Acid (C) -0.1 -0.6 +1.1 -0.7 +0.3
phage 24hr PHYTO6 MOI 1 -0.1 -1.2 +0.8 +0.1 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.1 +1.2 -1.7 +0.1 +1.1
carbon source Phenylacetic 5 mM (C) -0.4 +0.4 +1.0 +0.4 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.0 +0.6 -0.8 +0.2 +1.0
motility outer cut, LB soft agar motility assay +1.0 -0.2 +0.7 +0.2 +0.3
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.1 +1.1 +1.0 +0.0 -0.2
stress Paraquat dichloride 0.064 mg/ml +1.4 -0.4 +1.2 +0.0 -0.1
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.5 +0.5 +0.3 +0.2 +0.9
supernatant control Vogels_fungal_media; pH 5.5 +0.6 +0.5 +1.0 +0.4 +0.3
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