Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS35385 and BPHYT_RS35390 are separated by 150 nucleotidesBPHYT_RS35390 and BPHYT_RS35395 are separated by 14 nucleotidesBPHYT_RS35395 and BPHYT_RS35400 are separated by 4 nucleotidesBPHYT_RS35400 and BPHYT_RS35405 are separated by 404 nucleotides BPHYT_RS35385: BPHYT_RS35385 - GntR family transcriptional regulator, at 3,538,535 to 3,539,272 _RS35385 BPHYT_RS35390: BPHYT_RS35390 - MFS transporter, at 3,539,423 to 3,540,727 _RS35390 BPHYT_RS35395: BPHYT_RS35395 - membrane protein, at 3,540,742 to 3,541,611 _RS35395 BPHYT_RS35400: BPHYT_RS35400 - ABC transporter, at 3,541,616 to 3,543,280 _RS35400 BPHYT_RS35405: BPHYT_RS35405 - hypothetical protein, at 3,543,685 to 3,543,912 _RS35405
Group Condition BPHYT_RS35385 BPHYT_RS35390 BPHYT_RS35395 BPHYT_RS35400 BPHYT_RS35405
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -1.5 -0.4 -0.4 -1.0 -1.0
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -1.8 -0.1 -0.5 -0.8 -0.1
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -1.6 -0.6 -0.8 +0.0 +0.0
carbon source Protocatechuic Acid (C) -0.1 +0.2 -0.4 -2.0 -0.7
phage 24hr PHYTO10 MOI 10 -1.0 +0.1 -0.2 -0.8 -1.1
phage P. phyto corn MOI 0.1 -0.9 +0.2 -0.2 -0.9 -1.1
carbon source 2-Deoxy-D-ribonic lithium salt (C) -1.5 +0.4 -0.9 +0.5 -1.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.5 -0.3 -0.1 -2.1 +0.3
phage 24hr PHYTO3 MOI 10 -1.6 +0.2 +0.2 -0.8 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.5 +0.4 -0.6 -1.5 -0.2
phage 36hr PHYTO2 MOI 1 -0.4 +0.1 +0.2 -2.1 -0.1
phage P. phyto corn MOI 1 -1.6 +0.4 -0.2 +0.1 -0.8
carbon source Suberic (C) +0.3 -0.2 -0.8 -0.4 -1.0
phage 24hr PHYTO6 MOI 10 +0.5 +0.2 -0.2 -1.8 -0.8
phage 36hr PHYTO11 MOI 0.1 +0.5 -0.0 -0.1 -1.4 -0.8
motility inner cut, LB soft agar motility assay -0.9 +0.3 +0.3 -0.6 -0.9
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.5 -0.1 -0.6 -0.1 +0.7
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.6 +0.6 -0.3 +0.1 -1.4
phage 24hr PHYTO3 MOI 10 +0.5 -0.4 -0.6 +0.0 -1.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.9 +0.1 +0.0 +0.8 -1.0
phage P. phyto PHYTO2 MOI 1 -0.6 +0.2 -0.1 -1.2 +0.9
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days -0.7 -0.1 -0.2 -0.8 +1.3
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) -1.1 +0.2 +0.1 -0.5 +1.1
carbon source D-Glucose 20 mM (C) -1.6 +0.2 +0.1 +0.6 +0.6
carbon source L-Glutamine (C) -0.8 -0.2 -0.4 +0.8 +0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +0.7 +0.5 +0.2 -1.0 +0.2
supernatant control Vogels_fungal_media; pH 5.5 +0.3 -0.7 +0.2 -0.3 +1.3
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days -0.8 -0.2 +0.0 +0.1 +1.7
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 -0.1 -0.4 +0.6 +1.6
phage 24hr PHYTO4 MOI 10 +1.1 +0.1 +0.0 +0.7 +0.8
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