Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS35325 and BPHYT_RS35330 are separated by 74 nucleotidesBPHYT_RS35330 and BPHYT_RS35335 are separated by 89 nucleotidesBPHYT_RS35335 and BPHYT_RS35340 are separated by 40 nucleotidesBPHYT_RS35340 and BPHYT_RS35345 overlap by 4 nucleotides BPHYT_RS35325: BPHYT_RS35325 - amidohydrolase, at 3,523,822 to 3,525,207 _RS35325 BPHYT_RS35330: BPHYT_RS35330 - purine permease, at 3,525,282 to 3,526,622 _RS35330 BPHYT_RS35335: BPHYT_RS35335 - cobalamin biosynthesis protein, at 3,526,712 to 3,527,794 _RS35335 BPHYT_RS35340: BPHYT_RS35340 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 3,527,835 to 3,528,425 _RS35340 BPHYT_RS35345: BPHYT_RS35345 - decarboxylase UbiD, at 3,528,422 to 3,529,816 _RS35345
Group Condition BPHYT_RS35325 BPHYT_RS35330 BPHYT_RS35335 BPHYT_RS35340 BPHYT_RS35345
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 N.D. -0.2 -0.7 -1.2
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.3 N.D. -0.2 -0.8 -0.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 N.D. +0.3 -0.9 -0.9
nutrient Min media with glucose -0.3 N.D. -0.3 -0.4 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.4 N.D. -0.7 -0.4 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.6 N.D. -1.0 +0.3 +0.2
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.8 N.D. -0.4 -0.8 -0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.9 N.D. -0.2 -0.3 +0.4
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.8 N.D. -0.0 -0.3 -1.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 N.D. -0.6 -0.5 +0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 N.D. -0.6 -0.6 +0.2
phage 24hr PHYTO MOI 0.1 +0.4 N.D. -0.2 -1.0 +0.1
carbon source Phenylacetic 5 mM (C) +0.2 N.D. -0.5 +0.4 -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +0.4 N.D. +0.4 -0.8 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 N.D. -0.8 +0.8 -0.5
phage 36hr PHYTO2 MOI 1 +0.4 N.D. +0.7 -0.3 -0.7
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.1 N.D. +0.6 -1.0 +0.3
nitrogen source L-Proline (N) -0.4 N.D. -0.4 +0.2 +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.2 N.D. +0.4 -0.6 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.0 N.D. -0.7 +0.9 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.0 N.D. -0.6 +0.4 +0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 N.D. -0.6 +0.2 +1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 N.D. -0.6 +0.0 +0.8
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.7 N.D. +0.3 +0.6 +0.5
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days +0.0 N.D. +1.0 -0.4 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.5 N.D. +0.6 +0.1 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.4 N.D. +0.0 +0.6 +0.7
phage 24hr PHYTO2 MOI 0.1 +0.3 N.D. +0.6 -0.5 +0.6
phage 36hr PHYTO10 MOI 10 +0.3 N.D. +0.8 -0.3 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 N.D. -0.2 +0.3 +0.9
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