Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS35225 and BPHYT_RS35230 are separated by 36 nucleotidesBPHYT_RS35230 and BPHYT_RS35235 are separated by 183 nucleotidesBPHYT_RS35235 and BPHYT_RS35240 are separated by 57 nucleotidesBPHYT_RS35240 and BPHYT_RS35245 are separated by 128 nucleotides BPHYT_RS35225: BPHYT_RS35225 - RNA polymerase subunit sigma-24, at 3,506,519 to 3,507,793 _RS35225 BPHYT_RS35230: BPHYT_RS35230 - type 11 methyltransferase, at 3,507,830 to 3,508,519 _RS35230 BPHYT_RS35235: BPHYT_RS35235 - amino acid transporter, at 3,508,703 to 3,509,320 _RS35235 BPHYT_RS35240: BPHYT_RS35240 - membrane protein, at 3,509,378 to 3,509,956 _RS35240 BPHYT_RS35245: BPHYT_RS35245 - hypothetical protein, at 3,510,085 to 3,510,261 _RS35245
Group Condition BPHYT_RS35225 BPHYT_RS35230 BPHYT_RS35235 BPHYT_RS35240 BPHYT_RS35245
carbon source Phenylacetic 5 mM (C) +0.2 -3.8 +0.5 +0.2 -0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -0.5 -0.9 +0.1 -0.1 -1.7
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.3 -1.6 +0.3 -0.4 -1.4
phage 24hr PHYTO2 MOI 0.1 +0.3 -0.8 -1.7 -0.3 -0.2
temperature LB, 23C +0.1 -1.2 -0.5 +0.1 -1.0
phage 36hr PHYTO1 MOI 1 -0.3 -0.6 -1.6 -0.3 +0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.4 -0.6 -0.3 -0.5 -1.5
nitrogen source L-Lysine (N) +0.2 -2.0 -0.3 -0.1 +0.1
supernatant control Vogels_fungal_media; pH 5.5 -0.3 +0.5 -0.4 -0.1 -1.6
carbon source D-Glucose (C) -0.3 +1.0 -1.5 -0.0 -0.9
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.1 -2.7 -0.0 +0.4 +0.5
phage 24hr PHYTO4 MOI 10 +0.4 -0.9 -1.3 +0.0 +0.4
phage P. phyto PHYTO2 MOI 1 +0.4 -0.7 -1.0 -0.4 +0.6
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.5 +0.0 +0.5 -0.5 -1.6
motility outer cut, LB soft agar motility assay +0.0 +1.3 -0.6 -0.4 -0.8
phage 36hr PHYTO11 MOI 0.1 +0.1 +0.9 -1.2 -0.5 +0.2
supernatant Supernatant; Trichoderma atroviride IMI tmk3mutant grown in Vogels_fungal_media, filtered; 0.8X +0.0 +1.2 -0.4 -0.2 -1.0
phage 24hr PHYTO7 MOI 1 +0.5 +0.7 -1.2 -0.0 -0.3
carbon source 2-Deoxy-D-Ribose (C) +0.2 +0.9 +0.3 -0.1 -1.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.3 +1.6 -0.2 +0.4 -2.0
phage 24hr PHYTO2 MOI 1 -0.6 +0.8 -0.3 -0.4 +0.8
phage 24hr PHYTO3 MOI 1 -0.2 -0.9 +0.6 -0.1 +0.9
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +1.0 +0.4 -0.9 -0.2 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.6 +1.1 -0.6 +0.4 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.4 +2.1 +0.1 +0.4 -1.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.6 +1.2 +0.4 +0.0 -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.2 +2.2 +0.0 -0.1 -0.3
carbon source 2-Deoxy-D-ribonic lithium salt (C) -0.3 +2.0 +0.3 +0.1 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 +1.9 +0.4 -0.2 +0.3
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 +1.5 +0.2 +0.3 +0.7
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