Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS34965 and BPHYT_RS34970 overlap by 8 nucleotidesBPHYT_RS34970 and BPHYT_RS34975 overlap by 8 nucleotidesBPHYT_RS34975 and BPHYT_RS34980 are separated by 40 nucleotidesBPHYT_RS34980 and BPHYT_RS34985 are separated by 26 nucleotides BPHYT_RS34965: BPHYT_RS34965 - short-chain dehydrogenase, at 3,443,595 to 3,444,392 _RS34965 BPHYT_RS34970: BPHYT_RS34970 - hypothetical protein, at 3,444,385 to 3,446,043 _RS34970 BPHYT_RS34975: BPHYT_RS34975 - L-aspartate dehydrogenase, at 3,446,036 to 3,446,866 _RS34975 BPHYT_RS34980: BPHYT_RS34980 - aldehyde dehydrogenase, at 3,446,907 to 3,448,397 _RS34980 BPHYT_RS34985: BPHYT_RS34985 - glyoxalase, at 3,448,424 to 3,449,389 _RS34985
Group Condition BPHYT_RS34965 BPHYT_RS34970 BPHYT_RS34975 BPHYT_RS34980 BPHYT_RS34985
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.6 -1.0 -0.3 -0.9
vitamin With vitamins t2 N.D. -0.5 -0.9 -0.1 -0.4
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. -2.1 +0.6 +0.1 -0.2
lb_dap plate control LB_DAP agar plate, outgrowth in LB N.D. +0.1 -0.7 -0.0 -1.0
carbon source 2-Deoxy-D-ribonic lithium salt (C) N.D. -0.9 +0.1 +0.1 -0.9
phage 36hr PHYTO6 MOI 0.1 N.D. +0.3 -0.3 -0.6 -0.5
nitrogen source NAG (N) N.D. +0.4 -1.2 -0.1 -0.1
nutrient L-Histidine N.D. -1.0 +0.5 +0.1 -0.5
phage 36hr PHYTO MOI 0.1 N.D. +0.6 -0.6 -0.2 -0.5
phage 24hr PHYTO3 MOI 10 N.D. -1.0 +0.7 -0.3 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. -1.0 +0.8 -0.8 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.6 +0.6 -0.0 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.7 -0.8 -0.0 -0.3
supernatant control Vogels_fungal_media; pH 5.5 N.D. +0.3 +1.0 -1.0 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.5 -0.6 -0.5 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X N.D. +0.9 +0.1 -0.4 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +1.4 -0.5 -0.8 +0.1
nophagecontrol 24hr Phyto Lib Only N.D. +0.7 +0.2 -0.2 -0.5
phage P. phyto PHYTO2 MOI 1 N.D. +0.2 +1.0 -0.2 -0.7
phage 24hr PHYTO2 MOI 1 N.D. +1.0 -0.4 +0.2 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -0.2 +1.4 -0.4 -0.3
nitrogen source L-Glutamic (N) N.D. -0.5 +0.8 -0.1 +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.7 +0.6 -0.2 -0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. +1.4 -0.4 -0.5 +0.5
carbon source Suberic (C) N.D. +0.2 +1.2 +0.2 -0.1
carbon source Phenylacetic 5 mM (C) N.D. +0.4 +0.7 +0.6 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.9 +1.0 +0.2 -0.6
motility outer cut, LB soft agar motility assay N.D. +0.6 +0.4 +0.3 +0.3
motility outer cut, LB soft agar motility assay N.D. +0.4 +1.3 +0.0 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.1 +1.3 +0.6 +0.1
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