Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS34920 and BPHYT_RS34925 are separated by 90 nucleotidesBPHYT_RS34925 and BPHYT_RS34930 are separated by 441 nucleotidesBPHYT_RS34930 and BPHYT_RS34935 are separated by 1 nucleotidesBPHYT_RS34935 and BPHYT_RS34940 are separated by 48 nucleotides BPHYT_RS34920: BPHYT_RS34920 - IclR family transcriptional regulator, at 3,436,110 to 3,437,084 _RS34920 BPHYT_RS34925: BPHYT_RS34925 - porin, at 3,437,175 to 3,438,248 _RS34925 BPHYT_RS34930: BPHYT_RS34930 - Rieske (2Fe-2S) protein, at 3,438,690 to 3,439,004 _RS34930 BPHYT_RS34935: BPHYT_RS34935 - hypothetical protein, at 3,439,006 to 3,439,317 _RS34935 BPHYT_RS34940: BPHYT_RS34940 - 3-phenylpropionate dioxygenase, at 3,439,366 to 3,440,460 _RS34940
Group Condition BPHYT_RS34920 BPHYT_RS34925 BPHYT_RS34930 BPHYT_RS34935 BPHYT_RS34940
carbon source Malonic (C) -2.0 -0.0 -0.3 N.D. -0.3
nitrogen source L-Lysine (N) -0.7 -0.2 +0.5 N.D. -1.9
carbon source succinate (C) -1.0 -0.1 -0.5 N.D. -0.8
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) -1.3 +0.2 -0.4 N.D. -0.6
r2a control R2A control -1.5 -0.0 -0.5 N.D. -0.2
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.8 -0.4 -0.6 N.D. -0.2
supernatant control Vogels_fungal_media; pH 5.5 -0.5 -0.1 -0.1 N.D. -1.2
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) -1.2 -0.0 -0.0 N.D. -0.7
supernatant control:fungal media R2A with Vogels_fungal_media 0.2X -1.5 +0.1 +0.0 N.D. -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.4 +0.4 -0.3 N.D. -0.4
phage P. phyto PHYTO2 MOI 0.1 -1.3 +0.2 -0.3 N.D. +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.5 +0.5 +0.0 N.D. -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 -0.3 -0.6 N.D. +0.9
phage 36hr PHYTO10 MOI 10 +0.6 +0.2 -1.0 N.D. +0.2
phage 36hr PHYTO6 MOI 0.1 +1.2 -0.1 -0.7 N.D. -0.4
phage 36hr PHYTO10 MOI 10 +1.1 +0.0 -1.2 N.D. +0.2
phage 36hr PHYTO8 MOI 10 +0.9 +0.3 -1.0 N.D. -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.9 +0.9 -0.2 N.D. +0.4
phage 36hr PHYTO1 MOI 1 +1.3 +0.1 -0.7 N.D. -0.1
phage 36hr PHYTO6 MOI 0.1 +1.2 +0.1 -0.7 N.D. +0.0
phage 24hr PHYTO2 MOI 0.1 +1.0 +0.0 -0.8 N.D. +0.4
nophagecontrol 36hr Phyto Lib Only +1.2 -0.3 -0.4 N.D. +0.3
phage 36hr PHYTO4 MOI 0.1 +0.5 +0.2 -0.7 N.D. +0.8
phage 24hr PHYTO1 MOI 10 +1.3 +0.2 -0.7 N.D. -0.0
phage 36hr PHYTO2 MOI 1 +1.3 +0.3 -0.9 N.D. +0.2
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.2 -0.2 +1.6 N.D. -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.9 -0.0 +0.9 N.D. -0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.0 -0.2 +0.3 N.D. +0.8
phage 24hr PHYTO4 MOI 10 +1.2 +0.2 +0.1 N.D. +0.5
carbon source Malonic (C) +1.6 +0.1 +0.2 N.D. +0.1
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