Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS34840 and BPHYT_RS34845 are separated by 137 nucleotidesBPHYT_RS34845 and BPHYT_RS34850 are separated by 214 nucleotidesBPHYT_RS34850 and BPHYT_RS34855 are separated by 118 nucleotidesBPHYT_RS34855 and BPHYT_RS34860 are separated by 562 nucleotides BPHYT_RS34840: BPHYT_RS34840 - hypothetical protein, at 3,421,747 to 3,422,310 _RS34840 BPHYT_RS34845: BPHYT_RS34845 - NAD-dependent dehydratase, at 3,422,448 to 3,423,458 _RS34845 BPHYT_RS34850: BPHYT_RS34850 - acylphosphatase, at 3,423,673 to 3,423,966 _RS34850 BPHYT_RS34855: BPHYT_RS34855 - methyltransferase, at 3,424,085 to 3,425,065 _RS34855 BPHYT_RS34860: BPHYT_RS34860 - chemotaxis protein, at 3,425,628 to 3,426,524 _RS34860
Group Condition BPHYT_RS34840 BPHYT_RS34845 BPHYT_RS34850 BPHYT_RS34855 BPHYT_RS34860
carbon source Malonic (C) N.D. N.D. -0.9 -0.4 -0.7
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) N.D. N.D. -1.1 -0.2 -0.6
phage 36hr PHYTO7 MOI 1 N.D. N.D. +0.0 -0.2 -1.5
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. N.D. -0.3 -0.3 -1.1
nophagecontrol 24hr Phyto Lib only N.D. N.D. +0.8 -0.4 -1.8
supernatant control Vogels_fungal_media 0.1X N.D. N.D. -1.0 +0.3 -0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB N.D. N.D. +0.5 -0.5 -1.2
phage 36hr PHYTO4 MOI 1 N.D. N.D. +0.6 -0.6 -1.2
phage 36hr PHYTO9 MOI 1 N.D. N.D. +0.5 +0.3 -1.9
phage 24hr PHYTO MOI 0.1 N.D. N.D. +0.3 +0.3 -1.6
motility outer cut, LB soft agar motility assay N.D. N.D. -1.1 -0.1 +0.6
nophagecontrol 36hr Phyto Lib Only N.D. N.D. +0.6 -0.2 -0.8
phage 36hr PHYTO6 MOI 0.1 N.D. N.D. +0.8 -0.3 -0.8
phage P. phyto PHYTO2 MOI 1 N.D. N.D. +0.1 +0.7 -1.0
phage 36hr PHYTO3 MOI 0.1 N.D. N.D. +0.7 +0.1 -0.9
phage 24hr PHYTO4 MOI 0.1 N.D. N.D. +1.1 -0.2 -1.0
phage 36hr PHYTO8 MOI 10 N.D. N.D. +0.9 -0.0 -0.9
phage 36hr PHYTO3 MOI 1 N.D. N.D. +0.8 +0.3 -1.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. N.D. +0.9 +0.0 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. +1.1 -0.7 -0.2
phage 36hr PHYTO8 MOI 10 N.D. N.D. +0.7 +0.4 -0.7
phage 24hr PHYTO3 MOI 10 N.D. N.D. +1.2 +0.0 -0.6
nitrogen source Ammonium chloride (N) N.D. N.D. +0.9 +0.4 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +1.2 +0.2 -0.6
nitrogen source L-Serine (N) N.D. N.D. +0.9 +0.4 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. +0.9 -0.3 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +1.5 +0.0 -0.3
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. +1.9 -0.2 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.6 +0.5 +0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +1.5 +0.4 -0.2
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