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Protein
Homologs
Fitness for 5 genes in
Burkholderia phytofirmans PsJN
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500 nt
BPHYT_RS34790 and BPHYT_RS34795 are separated by 3 nucleotides
BPHYT_RS34795 and BPHYT_RS34800 are separated by 145 nucleotides
BPHYT_RS34800 and BPHYT_RS34805 are separated by 138 nucleotides
BPHYT_RS34805 and BPHYT_RS34810 are separated by 2 nucleotides
BPHYT_RS34790: BPHYT_RS34790 - squalene--hopene cyclase, at 3,409,083 to 3,411,113
_RS34790
BPHYT_RS34795: BPHYT_RS34795 - hypothetical protein, at 3,411,117 to 3,411,848
_RS34795
BPHYT_RS34800: BPHYT_RS34800 - thioesterase, at 3,411,994 to 3,412,473
_RS34800
BPHYT_RS34805: BPHYT_RS34805 - ABC transporter, at 3,412,612 to 3,413,634
_RS34805
BPHYT_RS34810: BPHYT_RS34810 - RND transporter, at 3,413,637 to 3,416,252
_RS34810
Group
Condition
BPHYT
_RS34790
BPHYT
_RS34795
BPHYT
_RS34800
BPHYT
_RS34805
BPHYT
_RS34810
stress
Bacitracin 0.25 mg/ml
N.D.
N.D.
+0.0
-5.2
-7.0
pH
LB, pH4
N.D.
N.D.
-0.1
-5.5
-5.2
pH
LB, pH4
N.D.
N.D.
+0.1
-4.1
-5.2
stress
Bacitracin 0.125 mg/ml
N.D.
N.D.
-0.0
-3.1
-5.1
stress
Lomefloxacin 0.001 mM
N.D.
N.D.
+0.4
-3.4
-3.4
phage
PHYTO3 MOI 0.1
N.D.
N.D.
+0.7
-1.9
-4.3
phage
36hr PHYTO8 MOI 10
N.D.
N.D.
-0.6
-0.6
-3.7
phage
36hr PHYTO3 MOI 1
N.D.
N.D.
-0.3
-0.5
-4.1
phage
24hr PHYTO2 MOI 0.1
N.D.
N.D.
-0.2
-0.6
-4.0
stress
Cephalothin 2 mg/ml
N.D.
N.D.
+0.2
-2.4
-2.6
phage
36hr PHYTO2 MOI 1
N.D.
N.D.
-0.9
-2.0
-1.9
phage
24hr PHYTO3 MOI 10
N.D.
N.D.
+0.2
-1.4
-3.2
phage
24hr PHYTO3 MOI 10
N.D.
N.D.
-0.5
-1.1
-2.6
phage
36hr PHYTO2 MOI 1
N.D.
N.D.
-0.6
-0.8
-2.5
phage
36hr PHYTO3 MOI 1
N.D.
N.D.
-0.0
-1.0
-2.8
phage
24hr PHYTO11 MOI 1
N.D.
N.D.
-0.3
-0.7
-2.7
motility
outer cut, LB soft agar motility assay
N.D.
N.D.
+0.1
-1.6
-2.1
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X
N.D.
N.D.
-0.3
-2.3
-1.0
phage
P. phyto PHYTO2 MOI 1
N.D.
N.D.
-0.1
-1.8
-1.7
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X
N.D.
N.D.
-0.2
-1.8
-1.5
phage
36hr PHYTO8 MOI 10
N.D.
N.D.
-0.1
-1.5
-1.8
phage
24hr PHYTO7 MOI 1
N.D.
N.D.
-0.2
-0.8
-2.4
motility
inner cut, LB soft agar motility assay
N.D.
N.D.
-0.4
-1.5
-1.4
in planta
Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
N.D.
N.D.
+0.1
-1.3
-2.0
stress
Nickel (II) chloride 0.5 mM
N.D.
N.D.
-0.0
-1.1
-2.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days
N.D.
N.D.
-1.2
-0.7
-1.3
stress
Polymyxin B sulfate 0.0625 mg/ml
N.D.
N.D.
+0.1
-1.5
-1.7
motility
inner cut, LB soft agar motility assay
N.D.
N.D.
+0.3
-1.9
-1.6
phage
36hr PHYTO11 MOI 1
N.D.
N.D.
+0.3
-0.6
-2.8
stress
A22 0.005 mg/ml
N.D.
N.D.
+0.3
-1.1
-1.9
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