Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS34505 and BPHYT_RS34510 are separated by 307 nucleotidesBPHYT_RS34510 and BPHYT_RS34515 are separated by 12 nucleotidesBPHYT_RS34515 and BPHYT_RS34520 are separated by 56 nucleotidesBPHYT_RS34520 and BPHYT_RS34525 overlap by 4 nucleotides BPHYT_RS34505: BPHYT_RS34505 - hypothetical protein, at 3,348,489 to 3,349,061 _RS34505 BPHYT_RS34510: BPHYT_RS34510 - sodium:proton antiporter, at 3,349,369 to 3,350,955 _RS34510 BPHYT_RS34515: BPHYT_RS34515 - MFS transporter, at 3,350,968 to 3,352,299 _RS34515 BPHYT_RS34520: BPHYT_RS34520 - hydroxymethylglutaryl-CoA lyase, at 3,352,356 to 3,353,312 _RS34520 BPHYT_RS34525: BPHYT_RS34525 - carnitine dehydratase, at 3,353,309 to 3,354,502 _RS34525
Group Condition BPHYT_RS34505 BPHYT_RS34510 BPHYT_RS34515 BPHYT_RS34520 BPHYT_RS34525
phage 36hr PHYTO4 MOI 1 -0.5 -0.2 -0.2 -0.3 -0.5
carbon source Suberic (C) -0.7 -0.2 +0.2 -0.5 -0.5
phage 24hr PHYTO1 MOI 10 -0.1 -0.6 -0.1 -0.1 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -1.0 -0.1 +0.1 +0.1 -0.6
nitrogen source L-Lysine (N) -1.7 -0.2 +0.4 -0.0 +0.1
carbon source Tween 20 (C) -1.1 +0.1 +0.2 -0.4 -0.1
phage P. phyto PHYTO2 MOI 1 -1.1 -0.2 -0.1 -0.0 +0.3
phage 24hr PHYTO3 MOI 10 +0.4 -0.6 +0.0 -0.1 -0.8
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) -0.5 -0.1 -0.3 +0.5 -0.6
motility inner cut, LB soft agar motility assay -0.5 +0.0 -0.6 -0.3 +0.4
phage 36hr PHYTO1 MOI 10 +0.4 -0.2 -0.3 -0.3 -0.4
phage 24hr PHYTO4 MOI 0.1 +0.2 -0.4 -0.3 +0.2 -0.6
phage 36hr PHYTO8 MOI 0.1 +0.8 -0.4 -0.6 -0.1 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.2 +0.2 +0.3 +0.3 -1.1
phage 24hr PHYTO3 MOI 10 +0.7 +0.1 +0.2 -0.2 -1.3
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.2 +0.5 +0.1 -0.2 -0.9
phage 24hr PHYTO6 MOI 10 +0.4 +0.3 -0.0 -0.5 -0.4
phage 24hr PHYTO9 MOI 10 +0.8 -0.4 +0.0 -0.5 +0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.9 +0.0 +0.2 +0.6 -0.0
carbon source Phenylacetic 5 mM (C) +0.7 -0.2 -0.2 +0.3 -0.6
carbon source pyruvate (C) +0.6 +0.3 +0.0 -0.5 -0.4
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.7 -0.1 +0.1 +0.3 -0.5
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.7 +0.2 +0.1 -0.4 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.5 +0.0 +0.3 -0.4 -0.1
supernatant control Vogels_fungal_media; pH 5.5 +0.5 +0.2 -0.2 +0.8 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.6 -0.3 +0.7 +0.0 +0.6
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.0 +0.4 +0.6 +1.0 +0.9
nitrogen source L-Serine (N) +0.8 +0.2 +0.1 +0.4 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.7 +0.5 +0.4 +0.2 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +1.2 +0.6 +0.1 +0.6 -0.3
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