Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS34375 and BPHYT_RS34380 are separated by 90 nucleotidesBPHYT_RS34380 and BPHYT_RS34385 are separated by 138 nucleotidesBPHYT_RS34385 and BPHYT_RS34395 are separated by 1365 nucleotidesBPHYT_RS34395 and BPHYT_RS34400 are separated by 157 nucleotides BPHYT_RS34375: BPHYT_RS34375 - membrane protein, at 3,319,442 to 3,320,434 _RS34375 BPHYT_RS34380: BPHYT_RS34380 - thioredoxin reductase, at 3,320,525 to 3,322,270 _RS34380 BPHYT_RS34385: BPHYT_RS34385 - hypothetical protein, at 3,322,409 to 3,322,684 _RS34385 BPHYT_RS34395: BPHYT_RS34395 - multidrug transporter MatE, at 3,324,050 to 3,324,649 _RS34395 BPHYT_RS34400: BPHYT_RS34400 - hypothetical protein, at 3,324,807 to 3,325,064 _RS34400
Group Condition BPHYT_RS34375 BPHYT_RS34380 BPHYT_RS34385 BPHYT_RS34395 BPHYT_RS34400
phage 36hr PHYTO11 MOI 1 +0.3 +0.4 +0.2 +0.2 -3.8
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 +0.2 -0.1 -0.7 -2.0
carbon source D-Glucose (C) -0.2 -0.0 -0.1 -0.5 -1.7
phage 36hr PHYTO4 MOI 1 +0.2 -0.5 -0.4 -0.2 -1.5
carbon source Phenylacetic 5 mM (C) -0.2 +0.0 -0.2 -0.2 -1.7
carbon source Sebacic (C) -0.2 +0.1 +0.1 -0.3 -2.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.2 -0.7 -0.2 +0.2 -1.2
phage 24hr PHYTO6 MOI 10 +0.1 +0.0 +0.0 -0.7 -1.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.1 -0.2 +0.2 +0.3 -2.3
phage 24hr PHYTO7 MOI 10 +0.1 +0.6 -0.1 -0.6 -2.0
carbon source Suberic (C) -0.3 -0.5 -0.1 -0.4 -0.6
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.2 -0.3 +0.0 -0.2 -1.4
phage 24hr PHYTO9 MOI 0.1 +0.3 -0.2 -0.1 -0.6 -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +0.4 -0.0 -0.0 -0.7 -0.9
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.4 +0.2 -0.3 -0.4 -1.1
phage 24hr PHYTO3 MOI 1 +0.1 -0.2 -0.3 -0.7 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.5 -0.4 -0.0 -0.2 +0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 -0.3 -0.1 -0.5 +1.0
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 +0.0 -0.2 -0.6 +0.7
carbon source 2-Deoxy-D-ribonic lithium salt (C) -0.1 +0.9 -0.3 +0.4 -0.6
carbon source Malonic (C) +0.4 +0.4 +0.1 +0.2 -0.9
phage 36hr PHYTO4 MOI 0.1 +0.2 +0.3 -0.2 -0.8 +0.8
phage 24hr PHYTO2 MOI 0.1 +0.3 -0.2 -0.4 -0.3 +0.9
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.2 +0.6 +0.3 -0.8 +0.2
phage 36hr PHYTO2 MOI 1 -0.0 -0.1 -0.1 -0.5 +1.4
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.1 +0.6 -0.1 +0.7 -0.6
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.1 +0.7 -0.4 +0.3 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.1 +0.4 +0.1 -0.2 +1.0
phage 24hr PHYTO3 MOI 10 +0.2 +0.4 -0.3 +0.5 +0.6
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.0 +0.7 -0.1 +0.3 +0.8
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