Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS34090 and BPHYT_RS34095 are separated by 119 nucleotidesBPHYT_RS34095 and BPHYT_RS34100 are separated by 108 nucleotidesBPHYT_RS34100 and BPHYT_RS34105 are separated by 129 nucleotidesBPHYT_RS34105 and BPHYT_RS34110 are separated by 6 nucleotides BPHYT_RS34090: BPHYT_RS34090 - polyamine ABC transporter substrate-binding protein, at 3,245,820 to 3,247,097 _RS34090 BPHYT_RS34095: BPHYT_RS34095 - spermidine/putrescine ABC transporter substrate-binding protein, at 3,247,217 to 3,248,266 _RS34095 BPHYT_RS34100: BPHYT_RS34100 - spermidine/putrescine ABC transporter ATP-binding protein, at 3,248,375 to 3,249,472 _RS34100 BPHYT_RS34105: BPHYT_RS34105 - decarboxylase, at 3,249,602 to 3,251,149 _RS34105 BPHYT_RS34110: BPHYT_RS34110 - hypothetical protein, at 3,251,156 to 3,254,899 _RS34110
Group Condition BPHYT_RS34090 BPHYT_RS34095 BPHYT_RS34100 BPHYT_RS34105 BPHYT_RS34110
nitrogen source L-Serine (N) +0.1 -0.2 -2.3 -0.5 +0.2
phage 24hr PHYTO2 MOI 1 +0.0 -0.5 -0.9 +0.0 -0.5
phage P. phyto corn MOI 1 +0.0 -0.4 -1.1 -0.1 -0.0
phage 24hr PHYTO2 MOI 0.1 +0.1 -0.6 -0.7 +0.2 -0.3
phage 36hr PHYTO10 MOI 10 -0.2 -0.5 -0.7 +0.4 -0.2
nophagecontrol 24hr Phyto Lib Only +0.2 -0.4 -0.8 +0.2 -0.3
phage 36hr PHYTO2 MOI 1 -0.2 -0.1 -0.6 +0.6 -0.6
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.6 -0.2 -1.1 -0.1 +0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 -0.4 -0.7 +0.5 -0.2
phage 36hr PHYTO11 MOI 1 -0.1 +0.2 -0.9 +0.4 -0.2
carbon source succinate (C) -0.4 -0.3 +0.2 -0.4 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.1 +0.3 -1.0 -0.2 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.4 +0.4 -0.5 -0.5 -0.2
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.1 -0.2 -0.8 +0.4 +0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +0.2 -0.6 +0.6 -0.2
phage 24hr PHYTO1 MOI 10 -0.1 -0.4 +0.3 +0.6 -0.3
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.0 -0.5 +0.9 -0.0 -0.2
phage 24hr PHYTO MOI 0.1 -0.2 -0.4 +0.7 +0.5 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.1 -0.7 +1.1 +0.3 +0.0
phage 36hr PHYTO3 MOI 1 -0.2 -0.0 +0.9 +0.4 -0.3
phage 36hr PHYTO2 MOI 1 -0.0 -0.1 +0.6 +0.7 -0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -0.2 +1.2 +0.2 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.1 +0.3 -0.1 +1.0 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.2 +1.1 +0.4 +0.4 -0.6
supernatant control Vogels_fungal_media 0.1X +0.5 +0.3 +0.9 -0.1 -0.0
carbon source 2-Deoxy-D-ribonic lithium salt (C) +0.2 +0.2 +0.8 +0.6 -0.0
supernatant control Vogels_fungal_media; pH 5.5 +0.6 +0.0 +1.2 +0.4 -0.3
supernatant control Vogels_fungal_media 0.1X +0.1 +0.2 +1.6 +0.3 -0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X +0.4 +0.8 +1.2 +0.2 +0.1
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +1.4 +1.1 +1.4 +0.9 +0.1
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