Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS34035 and BPHYT_RS34040 are separated by 21 nucleotidesBPHYT_RS34040 and BPHYT_RS34045 are separated by 10 nucleotidesBPHYT_RS34045 and BPHYT_RS34050 are separated by 3 nucleotidesBPHYT_RS34050 and BPHYT_RS34055 overlap by 4 nucleotides BPHYT_RS34035: BPHYT_RS34035 - type II and III secretion system outermembrane protein, secretin, at 3,235,195 to 3,236,880 _RS34035 BPHYT_RS34040: BPHYT_RS34040 - hypothetical protein, at 3,236,902 to 3,238,806 _RS34040 BPHYT_RS34045: BPHYT_RS34045 - secretion system protein F, at 3,238,817 to 3,239,665 _RS34045 BPHYT_RS34050: BPHYT_RS34050 - secretion system protein F, at 3,239,669 to 3,240,550 _RS34050 BPHYT_RS34055: BPHYT_RS34055 - hypothetical protein, at 3,240,547 to 3,240,897 _RS34055
Group Condition BPHYT_RS34035 BPHYT_RS34040 BPHYT_RS34045 BPHYT_RS34050 BPHYT_RS34055
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -2.3 +0.2 N.D. -0.5 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -2.1 -0.3 N.D. +0.2 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days -2.9 +0.2 N.D. +0.6 N.D.
nitrogen source L-Lysine (N) +0.5 -0.5 N.D. -1.6 N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -2.1 +0.1 N.D. +0.6 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.9 +0.2 N.D. -0.6 N.D.
vitamin No vitamins t1 -1.3 +0.3 N.D. -0.4 N.D.
carbon source Malonic (C) -0.6 +0.5 N.D. -0.9 N.D.
phage 24hr PHYTO3 MOI 10 -0.8 +0.4 N.D. -0.6 N.D.
phage P. phyto corn MOI 0.1 -1.5 +0.2 N.D. +0.4 N.D.
nophagecontrol 24hr Phyto Lib only -1.5 +0.2 N.D. +0.4 N.D.
carbon source Dodecandioic (C) -1.9 +0.9 N.D. +0.4 N.D.
carbon source 2-Deoxy-D-Ribose (C) -0.9 -0.1 N.D. +0.8 N.D.
phage 24hr PHYTO7 MOI 1 -0.9 +0.4 N.D. +0.5 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB +1.2 -0.7 N.D. -0.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.7 +0.4 N.D. +0.7 N.D.
nophagecontrol 36hr Phyto Lib Only +0.8 +0.4 N.D. -0.8 N.D.
phage P. phyto PHYTO2 MOI 1 +0.4 +0.9 N.D. -0.6 N.D.
phage 36hr PHYTO10 MOI 1 +1.4 +0.3 N.D. -0.8 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X +0.7 +0.9 N.D. -0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +1.0 +0.7 N.D. -0.3 N.D.
phage 36hr PHYTO6 MOI 0.1 +1.2 +0.6 N.D. -0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.5 +2.0 N.D. +0.0 N.D.
phage P. phyto PHYTO2 MOI 1 +0.9 -0.2 N.D. +0.9 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.9 -0.3 N.D. +1.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +1.4 N.D. +0.6 N.D.
phage 36hr PHYTO4 MOI 1 +1.2 +0.2 N.D. +0.5 N.D.
phage 36hr PHYTO6 MOI 10 +1.4 +0.9 N.D. -0.3 N.D.
motility outer cut, LB soft agar motility assay +1.1 +0.9 N.D. +0.2 N.D.
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +2.7 -0.3 N.D. +0.0 N.D.
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