Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS33970 and BPHYT_RS33975 are separated by 24 nucleotidesBPHYT_RS33975 and BPHYT_RS33980 are separated by 39 nucleotidesBPHYT_RS33980 and BPHYT_RS33985 are separated by 110 nucleotidesBPHYT_RS33985 and BPHYT_RS33990 overlap by 4 nucleotides BPHYT_RS33970: BPHYT_RS33970 - hypothetical protein, at 3,212,505 to 3,213,011 _RS33970 BPHYT_RS33975: BPHYT_RS33975 - 2-methylcitrate dehydratase, at 3,213,036 to 3,214,487 _RS33975 BPHYT_RS33980: BPHYT_RS33980 - aconitate hydratase, at 3,214,527 to 3,217,244 _RS33980 BPHYT_RS33985: BPHYT_RS33985 - hypothetical protein, at 3,217,355 to 3,218,434 _RS33985 BPHYT_RS33990: BPHYT_RS33990 - ATPase, at 3,218,431 to 3,218,898 _RS33990
Group Condition BPHYT_RS33970 BPHYT_RS33975 BPHYT_RS33980 BPHYT_RS33985 BPHYT_RS33990
carbon source Protocatechuic Acid (C) N.D. -0.2 -4.9 -0.0 -0.2
phage 36hr PHYTO2 MOI 1 N.D. -0.8 -0.0 -0.0 -4.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. -0.4 -1.7 +0.1 -2.7
carbon source Phenylacetic 5 mM (C) N.D. -0.4 +0.3 -0.2 -4.0
carbon source propionate (C) N.D. -0.5 -2.6 -0.1 -1.0
stress Paraquat dichloride 0.064 mg/ml N.D. -0.4 -1.8 +0.1 -1.5
carbon source Tween 20 (C) N.D. -0.2 +0.1 -0.1 -2.8
phage 24hr PHYTO3 MOI 0.1 N.D. -0.3 -0.5 -0.0 -1.8
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. -0.3 -0.4 +0.1 -1.9
nitrogen source L-Alanine (N) N.D. +0.2 -0.6 -0.0 -1.8
carbon source Azelaic (C) N.D. -0.1 -0.3 +0.1 -1.9
phage 36hr PHYTO1 MOI 10 N.D. -0.2 +0.1 -0.0 -1.9
phage 24hr PHYTO6 MOI 0.1 N.D. +0.3 -0.4 +0.0 -1.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -0.0 -0.3 -0.1 -1.4
stress Paraquat dichloride 0.032 mg/ml N.D. -0.5 -1.1 +0.0 -0.3
phage 36hr PHYTO6 MOI 1 N.D. -0.4 -0.4 -0.4 -0.7
carbon source Protocatechuic Acid (C) N.D. +0.1 -3.1 +0.1 +1.0
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. +0.2 -0.3 -0.1 -1.6
phage 24hr PHYTO2 MOI 1 N.D. -0.2 -0.9 +0.1 -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.1 -0.5 -0.1 -1.1
phage 24hr PHYTO4 MOI 10 N.D. -0.8 +0.1 +0.2 -0.9
carbon source Suberic (C) N.D. -0.1 +0.4 -0.0 -1.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB N.D. -0.5 -1.3 -0.0 +1.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.0 -0.9 -0.3 +0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.0 -0.5 +0.1 +1.2
phage 24hr PHYTO7 MOI 1 N.D. -0.3 -0.2 -0.1 +1.4
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. -0.1 -0.6 -0.0 +1.9
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) N.D. -0.1 +1.0 -0.3 +0.6
nitrogen source L-Serine (N) N.D. -0.0 -0.3 +0.1 +1.9
supernatant control Vogels_fungal_media; pH 5.5 N.D. +0.2 +0.3 +0.1 +1.5
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BPHYT_RS33970
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