Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS33595 and BPHYT_RS33600 are separated by 109 nucleotidesBPHYT_RS33600 and BPHYT_RS33605 are separated by 93 nucleotidesBPHYT_RS33605 and BPHYT_RS33610 are separated by 165 nucleotidesBPHYT_RS33610 and BPHYT_RS33615 are separated by 1 nucleotides BPHYT_RS33595: BPHYT_RS33595 - 3-mercaptopyruvate sulfurtransferase, at 3,121,613 to 3,122,482 _RS33595 BPHYT_RS33600: BPHYT_RS33600 - carboxymethylenebutenolidase, at 3,122,592 to 3,123,476 _RS33600 BPHYT_RS33605: BPHYT_RS33605 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase, at 3,123,570 to 3,124,919 _RS33605 BPHYT_RS33610: BPHYT_RS33610 - DNA polymerase I, at 3,125,085 to 3,127,826 _RS33610 BPHYT_RS33615: BPHYT_RS33615 - decarboxylase, at 3,127,828 to 3,128,571 _RS33615
Group Condition BPHYT_RS33595 BPHYT_RS33600 BPHYT_RS33605 BPHYT_RS33610 BPHYT_RS33615
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -0.3 +0.0 -0.2 -3.3 -1.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.1 +0.0 +0.0 -3.2 -2.3
supernatant control Vogels_fungal_media; pH 5.5 -0.0 +0.0 +0.1 -3.5 -1.9
carbon source L-Fucose (C) +0.2 -0.3 +0.0 -3.6 -1.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.0 -0.2 +0.4 -3.3 -1.0
nophagecontrol 24hr Phyto Lib Only -0.1 +0.4 -0.0 -0.7 -3.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.2 +0.1 -0.0 -3.4 -0.1
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.5 -0.3 -0.1 -2.8 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 +0.5 -0.1 -2.2 -1.8
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 +0.7 -0.4 -2.6 -1.0
nitrogen source L-Glutamic (N) -0.3 +0.1 +0.2 -2.4 -0.9
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 -0.0 +0.1 -2.7 -0.6
carbon source Suberic (C) -0.1 +0.4 -0.1 -0.8 -2.0
phage 24hr PHYTO10 MOI 10 -0.1 +0.4 -0.6 -1.4 -0.6
phage 24hr PHYTO2 MOI 0.1 -0.4 +0.5 -0.1 -1.0 -1.4
phage 36hr PHYTO11 MOI 1 -0.0 +0.4 -0.5 -2.0 -0.2
phage 24hr PHYTO2 MOI 1 -0.2 +0.7 -0.4 -2.2 -0.1
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -0.3 +0.6 +0.5 -2.1 -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.2 +0.6 -0.0 -2.3 -0.5
phage 24hr PHYTO4 MOI 0.1 -0.2 +0.7 -0.1 +0.7 -3.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.0 -0.3 +0.1 -2.7 +1.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.0 +0.5 +0.3 -1.5 -1.0
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 +0.0 +0.7 -3.0 +1.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 +0.3 +0.4 -2.0 -0.1
nitrogen source L-Lysine (N) +0.2 +0.5 +0.1 +0.7 -2.6
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) -0.2 +0.3 -0.1 -2.0 +1.3
phage 24hr PHYTO4 MOI 10 -0.3 +1.0 -0.6 -0.8 +1.1
carbon source Suberic (C) +0.2 +0.6 -0.2 +1.2 -0.8
carbon source 2-Deoxy-D-ribonic lithium salt (C) +0.4 +0.5 -0.1 +2.1 -0.8
carbon source Malonic (C) -0.4 +0.4 +0.6 +1.9 +0.3
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