Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS33590 and BPHYT_RS33595 are separated by 219 nucleotidesBPHYT_RS33595 and BPHYT_RS33600 are separated by 109 nucleotidesBPHYT_RS33600 and BPHYT_RS33605 are separated by 93 nucleotidesBPHYT_RS33605 and BPHYT_RS33610 are separated by 165 nucleotides BPHYT_RS33590: BPHYT_RS33590 - Rieske (2Fe-2S) protein, at 3,120,287 to 3,121,393 _RS33590 BPHYT_RS33595: BPHYT_RS33595 - 3-mercaptopyruvate sulfurtransferase, at 3,121,613 to 3,122,482 _RS33595 BPHYT_RS33600: BPHYT_RS33600 - carboxymethylenebutenolidase, at 3,122,592 to 3,123,476 _RS33600 BPHYT_RS33605: BPHYT_RS33605 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase, at 3,123,570 to 3,124,919 _RS33605 BPHYT_RS33610: BPHYT_RS33610 - DNA polymerase I, at 3,125,085 to 3,127,826 _RS33610
Group Condition BPHYT_RS33590 BPHYT_RS33595 BPHYT_RS33600 BPHYT_RS33605 BPHYT_RS33610
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.6 -0.2 +0.1 -0.0 -3.4
carbon source L-Fucose (C) -0.3 +0.2 -0.3 +0.0 -3.6
supernatant control Vogels_fungal_media; pH 5.5 -0.6 -0.0 +0.0 +0.1 -3.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -0.1 -0.3 +0.0 -0.2 -3.3
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.4 -0.5 -0.3 -0.1 -2.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.1 +0.0 -0.2 +0.4 -3.3
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 -0.3 +0.0 +0.7 -3.0
phage 24hr PHYTO2 MOI 1 -0.7 -0.2 +0.7 -0.4 -2.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 +0.0 -0.3 +0.1 -2.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.7 +0.1 +0.0 +0.0 -3.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 +0.0 +0.7 -0.4 -2.6
phage 24hr PHYTO6 MOI 0.1 -0.3 -0.3 +0.2 -0.5 -1.5
nitrogen source L-Glutamic (N) +0.1 -0.3 +0.1 +0.2 -2.4
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -0.9 -0.3 +0.6 +0.5 -2.1
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 +0.2 -0.0 +0.1 -2.7
phage 36hr PHYTO11 MOI 1 +0.1 -0.0 +0.4 -0.5 -2.0
phage 36hr PHYTO6 MOI 10 +0.2 -0.4 +0.4 +0.0 -1.8
phage 24hr PHYTO8 MOI 1 -0.5 -0.3 +0.7 -0.4 -1.0
phage 24hr PHYTO3 MOI 10 -0.6 -0.5 +0.6 +0.1 -1.0
phage 24hr PHYTO3 MOI 10 -0.7 -0.1 +0.9 -0.3 -1.2
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) +0.6 -0.2 +0.3 -0.1 -2.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.1 +0.1 +0.6 -0.4 -1.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.4 +0.2 +0.6 -0.0 -2.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 +0.2 +0.5 -0.1 -2.2
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.5 -0.2 +0.5 -0.9 -0.7
phage 24hr PHYTO4 MOI 10 +0.1 -0.3 +1.0 -0.6 -0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 +0.3 +0.3 +0.4 -2.0
phage 36hr PHYTO8 MOI 10 -0.3 -0.2 +0.6 -0.7 +1.2
carbon source Malonic (C) -0.3 -0.4 +0.4 +0.6 +1.9
carbon source 2-Deoxy-D-ribonic lithium salt (C) +0.5 +0.4 +0.5 -0.1 +2.1
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