Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS33515 and BPHYT_RS33520 are separated by 948 nucleotidesBPHYT_RS33520 and BPHYT_RS33525 are separated by 196 nucleotidesBPHYT_RS33525 and BPHYT_RS33530 are separated by 416 nucleotidesBPHYT_RS33530 and BPHYT_RS33535 are separated by 153 nucleotides BPHYT_RS33515: BPHYT_RS33515 - porin, at 3,104,579 to 3,105,712 _RS33515 BPHYT_RS33520: BPHYT_RS33520 - hypothetical protein, at 3,106,661 to 3,106,843 _RS33520 BPHYT_RS33525: BPHYT_RS33525 - hypothetical protein, at 3,107,040 to 3,107,246 _RS33525 BPHYT_RS33530: BPHYT_RS33530 - tRNA-Met, at 3,107,663 to 3,107,750 _RS33530 BPHYT_RS33535: BPHYT_RS33535 - RNA polymerase sigma factor RpoD, at 3,107,904 to 3,109,949 _RS33535
Group Condition BPHYT_RS33515 BPHYT_RS33520 BPHYT_RS33525 BPHYT_RS33530 BPHYT_RS33535
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.1 N.D. +0.1 N.D. -7.3
motility outer cut, LB soft agar motility assay -4.0 N.D. +0.2 N.D. -2.5
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.3 N.D. +0.9 N.D. -6.2
motility outer cut, LB soft agar motility assay -4.1 N.D. +1.0 N.D. -2.6
phage PHYTO3 MOI 0.1 -3.8 N.D. -0.8 N.D. -0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus -3.4 N.D. +0.1 N.D. -1.7
motility inner cut, LB soft agar motility assay -3.4 N.D. +0.3 N.D. -1.8
motility inner cut, LB soft agar motility assay -3.4 N.D. +0.6 N.D. -2.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -3.4 N.D. +0.0 N.D. -1.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.7 N.D. -0.2 N.D. -1.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.8 N.D. -0.1 N.D. -2.6
motility inner cut, LB soft agar motility assay -3.0 N.D. +0.5 N.D. -1.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.5 N.D. -0.3 N.D. -2.2
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.4 N.D. +0.1 N.D. -4.4
carbon source Sebacic (C) +0.1 N.D. +0.1 N.D. -4.0
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) -0.9 N.D. -0.0 N.D. -2.8
motility outer cut, LB soft agar motility assay -2.6 N.D. +0.7 N.D. -1.8
in planta Plant=Zea_mays; PlantTreatment=None; sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=15_days +0.0 N.D. -0.0 N.D. -3.7
carbon source Suberic (C) -0.4 N.D. -0.3 N.D. -3.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.9 N.D. +0.3 N.D. -1.8
carbon source Sebacic (C) +0.1 N.D. -0.1 N.D. -3.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.9 N.D. +0.3 N.D. -1.7
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.1 N.D. +0.3 N.D. -2.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.3 N.D. +0.3 N.D. -2.1
stress Bacitracin 0.125 mg/ml -3.0 N.D. +0.3 N.D. +1.0
stress Bacitracin 0.25 mg/ml -5.3 N.D. +0.7 N.D. +3.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +1.5 N.D. +0.4 N.D. +1.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +1.7 N.D. +0.5 N.D. +1.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +2.5 N.D. +0.3 N.D. +1.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +2.0 N.D. +0.7 N.D. +1.5
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