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Protein
Homologs
Fitness for 5 genes in
Burkholderia phytofirmans PsJN
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
BPHYT_RS33375 and BPHYT_RS33380 are separated by 49 nucleotides
BPHYT_RS33380 and BPHYT_RS33385 are separated by 50 nucleotides
BPHYT_RS33385 and BPHYT_RS33390 are separated by 18 nucleotides
BPHYT_RS33390 and BPHYT_RS33395 are separated by 571 nucleotides
BPHYT_RS33375: BPHYT_RS33375 - membrane protein, at 3,070,979 to 3,072,196
_RS33375
BPHYT_RS33380: BPHYT_RS33380 - 2-nitropropane dioxygenase, at 3,072,246 to 3,073,295
_RS33380
BPHYT_RS33385: BPHYT_RS33385 - major facilitator superfamily protein, at 3,073,346 to 3,074,617
_RS33385
BPHYT_RS33390: BPHYT_RS33390 - phosphate ABC transporter substrate-binding protein, at 3,074,636 to 3,075,670
_RS33390
BPHYT_RS33395: BPHYT_RS33395 - metal-binding protein, at 3,076,242 to 3,077,099
_RS33395
Group
Condition
BPHYT
_RS33375
BPHYT
_RS33380
BPHYT
_RS33385
BPHYT
_RS33390
BPHYT
_RS33395
temperature
LB, 37C
-0.8
-1.5
-0.4
-0.2
-0.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.8
-0.5
-0.3
-0.6
-0.7
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.9
+0.0
-1.5
-0.4
-0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.9
-1.1
+0.4
-0.1
-0.5
phage
36hr PHYTO MOI 0.1
-0.1
-0.3
-2.2
+0.7
-0.2
phage
24hr PHYTO3 MOI 1
-0.4
-0.4
-1.8
+0.9
-0.3
phage
PHYTO3 MOI 0.1
-2.0
-0.0
+0.4
-0.4
+0.2
phage
36hr PHYTO4 MOI 1
-0.5
-0.3
-1.6
+0.9
-0.4
phage
36hr PHYTO7 MOI 0.1
-1.3
-0.1
-0.0
+0.7
-0.7
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB
+0.6
+1.0
-2.7
+0.1
-0.3
phage
24hr PHYTO9 MOI 10
-1.1
+0.5
-2.3
+1.2
+0.4
nophagecontrol
36hr Phyto Lib only
-0.2
+0.1
-1.9
+1.0
-0.3
phage
24hr PHYTO2 MOI 1
-1.1
+0.3
-0.5
+0.6
-0.5
phage
24hr PHYTO3 MOI 0.1
-1.4
+0.2
-0.7
+0.8
-0.1
phage
36hr PHYTO8 MOI 0.1
-1.6
-0.0
-0.3
+1.1
-0.3
phage
36hr PHYTO6 MOI 10
-0.1
+0.1
-0.8
+0.9
-1.0
phage
24hr PHYTO6 MOI 10
-0.7
+0.5
-1.8
+1.0
+0.1
phage
36hr PHYTO1 MOI 1
-0.7
-0.2
-0.5
+1.0
-0.6
phage
36hr PHYTO11 MOI 0.1
-0.6
-0.9
+0.4
+1.0
-0.4
phage
36hr PHYTO7 MOI 1
-0.2
-0.3
-0.9
+1.4
-0.2
in planta
Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days
+0.7
+0.4
-1.1
-0.4
+0.3
nophagecontrol
36hr Phyto Lib Only
-0.7
+0.5
-0.2
+0.9
-0.6
phage
24hr PHYTO4 MOI 0.1
-1.4
+0.5
+0.1
+0.9
+0.0
phage
24hr PHYTO11 MOI 1
-0.8
-0.3
+1.2
+0.7
-0.4
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.7
-0.5
+0.6
+0.6
+0.7
phage
24hr PHYTO9 MOI 0.1
+0.3
+0.8
-0.6
+0.7
-0.5
in planta
Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days
-0.3
+2.5
-2.3
+0.1
+0.9
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-1.0
+0.2
+1.6
+0.2
-0.3
replicable vector efficiency
Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
-0.7
-0.2
+1.1
+0.4
+0.5
in planta
Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days
-1.1
+1.3
+0.9
+0.9
+0.7
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