Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS33375 and BPHYT_RS33380 are separated by 49 nucleotidesBPHYT_RS33380 and BPHYT_RS33385 are separated by 50 nucleotidesBPHYT_RS33385 and BPHYT_RS33390 are separated by 18 nucleotidesBPHYT_RS33390 and BPHYT_RS33395 are separated by 571 nucleotides BPHYT_RS33375: BPHYT_RS33375 - membrane protein, at 3,070,979 to 3,072,196 _RS33375 BPHYT_RS33380: BPHYT_RS33380 - 2-nitropropane dioxygenase, at 3,072,246 to 3,073,295 _RS33380 BPHYT_RS33385: BPHYT_RS33385 - major facilitator superfamily protein, at 3,073,346 to 3,074,617 _RS33385 BPHYT_RS33390: BPHYT_RS33390 - phosphate ABC transporter substrate-binding protein, at 3,074,636 to 3,075,670 _RS33390 BPHYT_RS33395: BPHYT_RS33395 - metal-binding protein, at 3,076,242 to 3,077,099 _RS33395
Group Condition BPHYT_RS33375 BPHYT_RS33380 BPHYT_RS33385 BPHYT_RS33390 BPHYT_RS33395
temperature LB, 37C -0.8 -1.5 -0.4 -0.2 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.8 -0.5 -0.3 -0.6 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.9 +0.0 -1.5 -0.4 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.9 -1.1 +0.4 -0.1 -0.5
phage 36hr PHYTO MOI 0.1 -0.1 -0.3 -2.2 +0.7 -0.2
phage 24hr PHYTO3 MOI 1 -0.4 -0.4 -1.8 +0.9 -0.3
phage PHYTO3 MOI 0.1 -2.0 -0.0 +0.4 -0.4 +0.2
phage 36hr PHYTO4 MOI 1 -0.5 -0.3 -1.6 +0.9 -0.4
phage 36hr PHYTO7 MOI 0.1 -1.3 -0.1 -0.0 +0.7 -0.7
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.6 +1.0 -2.7 +0.1 -0.3
phage 24hr PHYTO9 MOI 10 -1.1 +0.5 -2.3 +1.2 +0.4
nophagecontrol 36hr Phyto Lib only -0.2 +0.1 -1.9 +1.0 -0.3
phage 24hr PHYTO2 MOI 1 -1.1 +0.3 -0.5 +0.6 -0.5
phage 24hr PHYTO3 MOI 0.1 -1.4 +0.2 -0.7 +0.8 -0.1
phage 36hr PHYTO8 MOI 0.1 -1.6 -0.0 -0.3 +1.1 -0.3
phage 36hr PHYTO6 MOI 10 -0.1 +0.1 -0.8 +0.9 -1.0
phage 24hr PHYTO6 MOI 10 -0.7 +0.5 -1.8 +1.0 +0.1
phage 36hr PHYTO1 MOI 1 -0.7 -0.2 -0.5 +1.0 -0.6
phage 36hr PHYTO11 MOI 0.1 -0.6 -0.9 +0.4 +1.0 -0.4
phage 36hr PHYTO7 MOI 1 -0.2 -0.3 -0.9 +1.4 -0.2
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.7 +0.4 -1.1 -0.4 +0.3
nophagecontrol 36hr Phyto Lib Only -0.7 +0.5 -0.2 +0.9 -0.6
phage 24hr PHYTO4 MOI 0.1 -1.4 +0.5 +0.1 +0.9 +0.0
phage 24hr PHYTO11 MOI 1 -0.8 -0.3 +1.2 +0.7 -0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.7 -0.5 +0.6 +0.6 +0.7
phage 24hr PHYTO9 MOI 0.1 +0.3 +0.8 -0.6 +0.7 -0.5
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.3 +2.5 -2.3 +0.1 +0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.0 +0.2 +1.6 +0.2 -0.3
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.7 -0.2 +1.1 +0.4 +0.5
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -1.1 +1.3 +0.9 +0.9 +0.7
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