Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS33275 and BPHYT_RS33280 are separated by 108 nucleotidesBPHYT_RS33280 and BPHYT_RS33285 overlap by 1 nucleotidesBPHYT_RS33285 and BPHYT_RS33290 overlap by 11 nucleotidesBPHYT_RS33290 and BPHYT_RS33295 overlap by 4 nucleotides BPHYT_RS33275: BPHYT_RS33275 - ABC transporter substrate-binding protein, at 3,047,328 to 3,049,070 _RS33275 BPHYT_RS33280: BPHYT_RS33280 - membrane protein, at 3,049,179 to 3,049,460 _RS33280 BPHYT_RS33285: BPHYT_RS33285 - sugar ABC transporter permease, at 3,049,460 to 3,050,269 _RS33285 BPHYT_RS33290: BPHYT_RS33290 - ABC transporter permease, at 3,050,259 to 3,051,155 _RS33290 BPHYT_RS33295: BPHYT_RS33295 - ABC transporter ATP-binding protein, at 3,051,152 to 3,052,240 _RS33295
Group Condition BPHYT_RS33275 BPHYT_RS33280 BPHYT_RS33285 BPHYT_RS33290 BPHYT_RS33295
phage 24hr PHYTO6 MOI 0.1 -0.2 -1.2 -0.1 -0.3 N.D.
phage P. phyto PHYTO2 MOI 1 +0.3 -1.6 +0.2 -0.4 N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.5 -1.5 +0.1 +0.5 N.D.
nophagecontrol 24hr Phyto Lib Only +0.1 -1.7 +0.1 +0.2 N.D.
phage 24hr PHYTO10 MOI 10 +0.3 -2.0 -0.0 +0.4 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.2 -1.0 -0.1 +0.2 N.D.
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.0 -1.2 +0.2 -0.1 N.D.
phage 36hr PHYTO6 MOI 0.1 +0.3 -0.9 -0.3 -0.1 N.D.
phage P. phyto PHYTO2 MOI 0.1 -0.6 -0.7 -0.1 +0.4 N.D.
phage 36hr PHYTO4 MOI 1 +0.4 -1.8 +0.2 +0.3 N.D.
nitrogen source L-Isoleucine (N) +0.3 -0.7 -0.4 -0.2 N.D.
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) +0.3 -1.0 +0.1 -0.2 N.D.
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.1 -1.1 +0.2 +0.0 N.D.
phage 24hr PHYTO1 MOI 10 +0.5 -1.2 -0.0 -0.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.4 -0.4 -0.2 +0.4 N.D.
phage 24hr PHYTO8 MOI 1 +0.1 -1.2 +0.2 +0.3 N.D.
phage 36hr PHYTO6 MOI 0.1 +0.2 -0.7 +0.3 -0.3 N.D.
phage 24hr PHYTO6 MOI 10 +0.5 -1.2 -0.1 +0.6 N.D.
phage 36hr PHYTO1 MOI 1 +0.4 -0.4 +0.2 -0.5 N.D.
phage 36hr PHYTO10 MOI 10 +0.4 -0.5 +0.5 -0.5 N.D.
motility inner cut, LB soft agar motility assay +0.2 +0.4 +0.1 -0.7 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.1 -0.7 +0.3 +0.6 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 -0.4 +0.7 +0.2 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 +1.0 -0.0 -0.2 N.D.
carbon source adipate (C) -0.5 +0.9 +0.0 +0.2 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 +1.0 +0.5 -0.4 N.D.
carbon source Dodecandioic (C) -0.3 +0.4 +0.4 +0.5 N.D.
phage 36hr PHYTO8 MOI 10 +0.3 +0.8 -0.1 +0.3 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X +0.3 +0.6 +0.3 +0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.6 +0.1 +0.2 +0.7 N.D.
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