Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS33080 and BPHYT_RS33095 are separated by 1797 nucleotidesBPHYT_RS33095 and BPHYT_RS33110 are separated by 1921 nucleotidesBPHYT_RS33110 and BPHYT_RS33115 are separated by 254 nucleotidesBPHYT_RS33115 and BPHYT_RS33120 are separated by 97 nucleotides BPHYT_RS33080: BPHYT_RS33080 - hypothetical protein, at 3,000,359 to 3,000,769 _RS33080 BPHYT_RS33095: BPHYT_RS33095 - hypothetical protein, at 3,002,567 to 3,002,755 _RS33095 BPHYT_RS33110: BPHYT_RS33110 - chloroperoxidase, at 3,004,677 to 3,005,663 _RS33110 BPHYT_RS33115: BPHYT_RS33115 - small mechanosensitive ion channel protein MscS, at 3,005,918 to 3,007,375 _RS33115 BPHYT_RS33120: BPHYT_RS33120 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase, at 3,007,473 to 3,008,681 _RS33120
Group Condition BPHYT_RS33080 BPHYT_RS33095 BPHYT_RS33110 BPHYT_RS33115 BPHYT_RS33120
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -0.8 -0.8 -1.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. -0.1 -0.8 -1.2
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. N.D. -0.4 -0.5 -1.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. -0.1 -0.4 -1.5
phage 24hr PHYTO3 MOI 10 N.D. N.D. -0.1 -0.7 -1.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -0.1 -1.1 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. N.D. -0.7 -0.6 -0.7
carbon source Suberic (C) N.D. N.D. -0.4 -0.3 -1.2
carbon source Phenylacetic 5 mM (C) N.D. N.D. -1.1 +0.5 -1.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -0.8 -0.6 -0.3
phage 24hr PHYTO11 MOI 0.1 N.D. N.D. +0.7 -0.1 -2.1
nitrogen source L-Glutamic (N) N.D. N.D. +0.4 -0.4 -1.4
phage 36hr PHYTO6 MOI 0.1 N.D. N.D. -0.6 +0.3 -1.1
phage 36hr PHYTO10 MOI 10 N.D. N.D. +0.1 +0.3 -1.7
phage 36hr PHYTO4 MOI 1 N.D. N.D. +0.5 -0.6 -1.0
nophagecontrol 36hr Phyto Lib only N.D. N.D. -0.2 +0.3 -1.1
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. N.D. +0.7 -0.8 -0.8
carbon source Tween 20 (C) N.D. N.D. +0.5 -0.4 -0.8
phage 24hr PHYTO2 MOI 0.1 N.D. N.D. +0.7 -0.4 -0.9
phage 36hr PHYTO1 MOI 10 N.D. N.D. +0.1 +0.7 -0.9
supernatant control Vogels_fungal_media; pH 5.5 N.D. N.D. +0.9 +0.1 -0.9
phage 24hr PHYTO1 MOI 10 N.D. N.D. +0.6 +0.4 -0.8
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -0.4 +1.1 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +1.3 -0.6 -0.3
phage 36hr PHYTO8 MOI 10 N.D. N.D. +0.9 +0.4 -0.9
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. +1.4 +0.3 -1.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. N.D. -0.5 +1.1 +0.5
phage 36hr PHYTO6 MOI 10 N.D. N.D. +0.8 -0.3 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +1.0 -0.1 +0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +3.9 -0.9 -0.5
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