Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS33040 and BPHYT_RS33045 are separated by 395 nucleotidesBPHYT_RS33045 and BPHYT_RS33050 are separated by 96 nucleotidesBPHYT_RS33050 and BPHYT_RS33055 are separated by 38 nucleotidesBPHYT_RS33055 and BPHYT_RS33060 are separated by 466 nucleotides BPHYT_RS33040: BPHYT_RS33040 - hypothetical protein, at 2,990,222 to 2,990,440 _RS33040 BPHYT_RS33045: BPHYT_RS33045 - response regulator receiver protein, at 2,990,836 to 2,991,213 _RS33045 BPHYT_RS33050: BPHYT_RS33050 - LuxR family transcriptional regulator, at 2,991,310 to 2,991,987 _RS33050 BPHYT_RS33055: BPHYT_RS33055 - hypothetical protein, at 2,992,026 to 2,993,654 _RS33055 BPHYT_RS33060: BPHYT_RS33060 - hypothetical protein, at 2,994,121 to 2,994,888 _RS33060
Group Condition BPHYT_RS33040 BPHYT_RS33045 BPHYT_RS33050 BPHYT_RS33055 BPHYT_RS33060
phage 36hr PHYTO8 MOI 10 +0.2 -1.6 -1.9 -0.2 -1.5
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.8 -0.6 -0.9 +0.2 -3.6
phage 24hr PHYTO11 MOI 1 +0.1 -1.7 -0.5 -0.8 -1.0
phage 24hr PHYTO2 MOI 0.1 +0.7 -0.9 -1.2 +0.3 -2.2
carbon source Phenylacetic 5 mM (C) -0.2 -1.1 -0.8 +0.3 -1.3
phage P. phyto corn MOI 0.1 +0.8 -0.7 -0.3 -0.3 -2.7
phage 24hr PHYTO10 MOI 1 +0.3 -2.6 +0.0 +0.1 -0.8
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.1 -0.6 +0.5 -0.2 -2.7
phage 24hr PHYTO4 MOI 0.1 +0.3 -1.2 -0.6 -0.1 -1.2
phage 24hr PHYTO9 MOI 10 -0.0 -0.7 +0.3 -0.0 -2.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 -1.3 +0.6 -0.4 -1.1
phage 24hr PHYTO2 MOI 0.1 +0.2 -2.6 -0.4 +0.2 +0.3
phage PHYTO3 MOI 0.1 +0.4 -2.4 +0.6 +0.3 -1.0
nophagecontrol 24hr Phyto Lib Only +0.3 -2.7 +0.1 +0.1 +0.3
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.4 -0.8 +0.7 -0.4 -1.8
phage 24hr PHYTO6 MOI 0.1 +0.4 -2.6 -0.3 +0.2 +0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 -1.6 +0.1 +0.4 -0.8
nitrogen source L-Isoleucine (N) +0.7 -1.2 -0.8 +0.1 -0.3
phage 36hr PHYTO6 MOI 10 +0.0 -2.4 +0.4 +0.0 +0.5
phage 24hr PHYTO6 MOI 0.1 +0.2 -1.9 -0.2 -0.1 +0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -0.3 -1.5 +1.3 -0.3 -0.3
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.7 -1.2 -0.5 +0.5 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.4 +0.4 +0.4 -0.6 -1.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.0 -0.4 +0.6 +0.6 -1.4
nitrogen source L-Lysine (N) -0.3 +1.0 -0.4 +0.8 -1.6
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 -1.2 +0.7 +0.3 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.3 -1.4 +0.7 -0.1 +0.7
nitrogen source L-Glutamic (N) +0.2 +1.4 +0.4 +0.4 -1.2
vitamin No vitamins t1 +0.2 -0.8 +0.2 -0.5 +2.1
phage 36hr PHYTO1 MOI 10 +0.4 +1.0 +0.6 -0.3 +1.0
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