Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS32985 and BPHYT_RS32990 are separated by 286 nucleotidesBPHYT_RS32990 and BPHYT_RS32995 are separated by 48 nucleotidesBPHYT_RS32995 and BPHYT_RS33000 are separated by 69 nucleotidesBPHYT_RS33000 and BPHYT_RS33005 are separated by 95 nucleotides BPHYT_RS32985: BPHYT_RS32985 - hypothetical protein, at 2,979,118 to 2,980,053 _RS32985 BPHYT_RS32990: BPHYT_RS32990 - hypothetical protein, at 2,980,340 to 2,981,848 _RS32990 BPHYT_RS32995: BPHYT_RS32995 - hypothetical protein, at 2,981,897 to 2,983,363 _RS32995 BPHYT_RS33000: BPHYT_RS33000 - histidine biosynthesis protein, at 2,983,433 to 2,983,702 _RS33000 BPHYT_RS33005: BPHYT_RS33005 - AraC family transcriptional regulator, at 2,983,798 to 2,984,772 _RS33005
Group Condition BPHYT_RS32985 BPHYT_RS32990 BPHYT_RS32995 BPHYT_RS33000 BPHYT_RS33005
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.6 -0.8 -0.6 N.D. N.D.
carbon source Phenylacetic 5 mM (C) -0.4 -0.6 -0.9 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 +0.0 -0.6 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.4 -0.3 -0.5 N.D. N.D.
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X -0.7 +0.0 -0.6 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.9 -0.1 -0.2 N.D. N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.3 -0.2 -0.7 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 -0.7 -0.5 N.D. N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days -0.6 +0.2 -0.5 N.D. N.D.
lb_dap plate control LB_DAP agar plate, outgrowth in LB -0.6 -0.5 +0.2 N.D. N.D.
carbon source D-Glucose (C) +0.1 -0.8 -0.2 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -1.2 -0.1 +0.4 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.9 +0.4 -0.4 N.D. N.D.
phage 36hr PHYTO1 MOI 10 -0.7 +0.2 -0.2 N.D. N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days +0.4 -0.7 -0.2 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 +0.5 -0.3 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.6 -0.3 -0.7 N.D. N.D.
phage 24hr PHYTO2 MOI 0.1 -0.9 +0.2 +0.3 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.4 -0.6 +0.6 N.D. N.D.
motility outer cut, LB soft agar motility assay -0.2 +0.5 -0.5 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.7 +0.3 +0.4 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 -0.4 +0.7 N.D. N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.2 +0.7 -0.3 N.D. N.D.
nitrogen source L-Isoleucine (N) -0.1 +0.7 -0.3 N.D. N.D.
carbon source 2-Deoxy-D-ribonic lithium salt (C) +0.5 -0.6 +0.5 N.D. N.D.
motility outer cut, LB soft agar motility assay +0.5 +0.3 -0.3 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +0.8 -0.3 +0.3 N.D. N.D.
supernatant control Vogels_fungal_media; pH 5.5 +0.8 -0.1 +0.3 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 +0.6 +0.9 N.D. N.D.
carbon source Malonic (C) +1.0 +0.6 +0.4 N.D. N.D.
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