Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS32930 and BPHYT_RS32935 are separated by 57 nucleotidesBPHYT_RS32935 and BPHYT_RS32940 are separated by 86 nucleotidesBPHYT_RS32940 and BPHYT_RS32945 are separated by 341 nucleotidesBPHYT_RS32945 and BPHYT_RS32950 are separated by 21 nucleotides BPHYT_RS32930: BPHYT_RS32930 - NADPH:quinone reductase and related Zn-dependent oxidoreductase-like protein, at 2,968,454 to 2,968,900 _RS32930 BPHYT_RS32935: BPHYT_RS32935 - 3-ketoacyl-ACP reductase, at 2,968,958 to 2,969,704 _RS32935 BPHYT_RS32940: BPHYT_RS32940 - MFS transporter, at 2,969,791 to 2,970,540 _RS32940 BPHYT_RS32945: BPHYT_RS32945 - integrase, at 2,970,882 to 2,971,757 _RS32945 BPHYT_RS32950: BPHYT_RS32950 - transposase, at 2,971,779 to 2,972,948 _RS32950
Group Condition BPHYT_RS32930 BPHYT_RS32935 BPHYT_RS32940 BPHYT_RS32945 BPHYT_RS32950
phage 24hr PHYTO3 MOI 10 -0.8 -0.4 -1.2 N.D. N.D.
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.8 -0.5 -0.8 N.D. N.D.
phage 36hr PHYTO2 MOI 1 -1.1 -0.2 -0.8 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.6 -0.4 -0.7 N.D. N.D.
phage 24hr PHYTO9 MOI 10 -1.4 -0.1 -0.2 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.9 +0.3 -1.0 N.D. N.D.
carbon source propionate (C) -0.4 -0.1 -1.0 N.D. N.D.
carbon source Sebacic (C) -1.1 +0.1 -0.5 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.2 -0.0 -0.2 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days -1.0 +0.1 -0.5 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.2 +0.2 -0.4 N.D. N.D.
phage 36hr PHYTO7 MOI 1 -1.4 +0.2 +0.1 N.D. N.D.
carbon source D-Glucose (C) -0.5 +0.3 -0.9 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 +0.3 -0.9 N.D. N.D.
phage 36hr PHYTO7 MOI 0.1 -0.7 +0.3 -0.6 N.D. N.D.
nitrogen source L-Asparagine (N) -0.3 +0.3 -0.8 N.D. N.D.
phage 24hr PHYTO9 MOI 0.1 -0.9 +0.4 -0.2 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.6 +1.0 -1.1 N.D. N.D.
vitamin No vitamins t1 -1.0 +0.5 -0.1 N.D. N.D.
phage PHYTO3 MOI 0.1 -0.6 +0.7 -0.4 N.D. N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.8 -0.4 -0.6 N.D. N.D.
vitamin With vitamins t1 -0.8 +0.3 +0.4 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X +0.8 -0.4 -0.4 N.D. N.D.
motility inner cut, LB soft agar motility assay +0.8 -0.5 +0.2 N.D. N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.6 -0.4 +0.6 N.D. N.D.
supernatant control Vogels_fungal_media; pH 5.5 +0.6 -0.3 +0.5 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB +0.3 -0.3 +0.8 N.D. N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.8 +0.5 +0.2 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +1.4 +0.2 +0.3 N.D. N.D.
carbon source Malonic (C) +1.5 +0.1 +1.2 N.D. N.D.
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