Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS32885 and BPHYT_RS32890 are separated by 195 nucleotidesBPHYT_RS32890 and BPHYT_RS32895 are separated by 141 nucleotidesBPHYT_RS32895 and BPHYT_RS32900 are separated by 52 nucleotidesBPHYT_RS32900 and BPHYT_RS32905 are separated by 20 nucleotides BPHYT_RS32885: BPHYT_RS32885 - methyl-accepting chemotaxis protein, at 2,957,012 to 2,958,763 _RS32885 BPHYT_RS32890: BPHYT_RS32890 - LysR family transcriptional regulator, at 2,958,959 to 2,959,909 _RS32890 BPHYT_RS32895: BPHYT_RS32895 - sulfatase, at 2,960,051 to 2,961,481 _RS32895 BPHYT_RS32900: BPHYT_RS32900 - MFS transporter, at 2,961,534 to 2,962,835 _RS32900 BPHYT_RS32905: BPHYT_RS32905 - FAD-linked oxidase, at 2,962,856 to 2,964,247 _RS32905
Group Condition BPHYT_RS32885 BPHYT_RS32890 BPHYT_RS32895 BPHYT_RS32900 BPHYT_RS32905
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.4 N.D. +0.1 -0.5 -0.7
carbon source D,L-Lactate (C) -0.6 N.D. +0.1 -0.4 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.1 N.D. -0.0 +0.1 -1.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 N.D. +0.1 -0.6 -1.0
phage 36hr PHYTO2 MOI 1 -0.2 N.D. -0.4 +0.3 -0.9
carbon source Suberic (C) -0.7 N.D. -0.5 -0.3 +0.4
phage 24hr PHYTO2 MOI 1 -0.4 N.D. -0.4 +0.4 -0.7
phage 24hr PHYTO6 MOI 10 -0.5 N.D. -0.2 +0.4 -0.6
carbon source Phenylacetic 5 mM (C) -0.8 N.D. -0.2 -0.6 +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.1 N.D. +0.4 -0.1 -1.0
phage 24hr PHYTO11 MOI 1 -0.1 N.D. -0.3 +0.5 -0.9
phage P. phyto PHYTO2 MOI 1 +0.2 N.D. +0.3 -0.7 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.6 N.D. +0.1 -0.5 +0.4
phage 24hr PHYTO3 MOI 10 +0.3 N.D. -0.7 +0.4 -0.3
phage 36hr PHYTO8 MOI 0.1 +0.3 N.D. -0.6 +0.5 -0.3
phage 24hr PHYTO7 MOI 10 -0.2 N.D. -0.1 +0.9 -0.6
phage 24hr PHYTO9 MOI 0.1 +0.8 N.D. -0.1 +0.1 -0.5
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days +0.9 N.D. -0.2 +0.3 -0.6
phage 36hr PHYTO11 MOI 1 -0.2 N.D. -0.4 +0.7 +0.3
carbon source Tween 20 (C) -0.2 N.D. -0.3 +0.3 +0.8
nitrogen source L-Lysine (N) +0.1 N.D. +0.3 -0.5 +0.7
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.2 N.D. +0.3 +0.4 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 N.D. -0.1 +1.8 -0.7
supernatant control Vogels_fungal_media 0.1X +0.7 N.D. -0.1 +0.1 +0.8
motility outer cut, LB soft agar motility assay +0.7 N.D. -0.1 +0.6 +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +0.4 N.D. +0.4 +0.3 +0.6
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.7 N.D. +0.2 +0.5 +0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X +0.2 N.D. +0.3 +0.5 +0.9
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +0.6 N.D. +0.4 +0.5 +0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.3 N.D. +0.6 +0.8 +1.0
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