Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS32875 and BPHYT_RS32880 are separated by 22 nucleotidesBPHYT_RS32880 and BPHYT_RS32885 are separated by 651 nucleotidesBPHYT_RS32885 and BPHYT_RS32890 are separated by 195 nucleotidesBPHYT_RS32890 and BPHYT_RS32895 are separated by 141 nucleotides BPHYT_RS32875: BPHYT_RS32875 - GCN5 family acetyltransferase, at 2,954,950 to 2,955,762 _RS32875 BPHYT_RS32880: BPHYT_RS32880 - alanine acetyltransferase, at 2,955,785 to 2,956,360 _RS32880 BPHYT_RS32885: BPHYT_RS32885 - methyl-accepting chemotaxis protein, at 2,957,012 to 2,958,763 _RS32885 BPHYT_RS32890: BPHYT_RS32890 - LysR family transcriptional regulator, at 2,958,959 to 2,959,909 _RS32890 BPHYT_RS32895: BPHYT_RS32895 - sulfatase, at 2,960,051 to 2,961,481 _RS32895
Group Condition BPHYT_RS32875 BPHYT_RS32880 BPHYT_RS32885 BPHYT_RS32890 BPHYT_RS32895
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -3.1 -0.5 N.D. -0.3
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. -3.5 +0.4 N.D. +0.2
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 N.D. -2.4 -0.5 N.D. -0.1
phage P. phyto corn MOI 1 N.D. -2.0 -0.4 N.D. +0.0
phage 24hr PHYTO10 MOI 10 N.D. -1.5 -0.5 N.D. -0.2
phage 36hr PHYTO8 MOI 0.1 N.D. -1.6 +0.3 N.D. -0.6
lb_dap plate control LB_DAP agar plate, outgrowth in LB N.D. -2.2 +0.2 N.D. +0.1
nitrogen source Uridine (N) N.D. -1.5 -0.1 N.D. -0.4
carbon source Protocatechuic Acid (C) N.D. -1.5 -0.2 N.D. -0.1
motility inner cut, LB soft agar motility assay N.D. -1.8 +0.1 N.D. +0.1
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. -2.0 +0.5 N.D. -0.2
phage 36hr PHYTO9 MOI 1 N.D. -1.4 +0.3 N.D. -0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.4 -0.1 N.D. +0.3
motility outer cut, LB soft agar motility assay N.D. -1.5 +0.7 N.D. -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. -1.7 +0.3 N.D. +0.6
pH LB, pH7 N.D. -1.2 +0.4 N.D. +0.2
carbon source Phenylacetic 5 mM (C) N.D. +0.7 -0.8 N.D. -0.2
carbon source 2-Deoxy-D-ribonic lithium salt (C) N.D. +1.2 -0.3 N.D. -0.3
nitrogen source L-Serine (N) N.D. +1.4 -0.4 N.D. +0.0
carbon source adipate (C) N.D. +1.0 +0.5 N.D. -0.3
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +1.2 +0.5 N.D. -0.1
supernatant control Vogels_fungal_media; pH 5.5 N.D. +1.5 -0.1 N.D. +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +1.9 -0.3 N.D. +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +2.5 -0.3 N.D. -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +1.2 +0.5 N.D. +0.2
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. +1.8 +0.1 N.D. +0.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days N.D. +1.8 +0.4 N.D. -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +2.4 -0.0 N.D. -0.1
temperature LB 37C N.D. +2.9 -0.0 N.D. -0.3
temperature LB 37C N.D. +5.0 +0.0 N.D. -0.0
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