Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS32580 and BPHYT_RS32585 are separated by 17 nucleotidesBPHYT_RS32585 and BPHYT_RS32590 are separated by 800 nucleotidesBPHYT_RS32590 and BPHYT_RS32595 are separated by 88 nucleotidesBPHYT_RS32595 and BPHYT_RS32600 are separated by 451 nucleotides BPHYT_RS32580: BPHYT_RS32580 - tRNA-Ile, at 2,879,571 to 2,879,647 _RS32580 BPHYT_RS32585: BPHYT_RS32585 - 16S ribosomal RNA, at 2,879,665 to 2,881,259 _RS32585 BPHYT_RS32590: BPHYT_RS32590 - RNA polymerase sigma factor RpoD, at 2,882,060 to 2,884,057 _RS32590 BPHYT_RS32595: BPHYT_RS32595 - hypothetical protein, at 2,884,146 to 2,884,526 _RS32595 BPHYT_RS32600: BPHYT_RS32600 - photosystem reaction center subunit H, at 2,884,978 to 2,885,454 _RS32600
Group Condition BPHYT_RS32580 BPHYT_RS32585 BPHYT_RS32590 BPHYT_RS32595 BPHYT_RS32600
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. N.D. -1.3 -0.3
supernatant control Vogels_fungal_media; pH 5.5 N.D. N.D. N.D. -0.7 -0.9
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. N.D. -1.2 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. N.D. N.D. -0.5 -0.9
carbon source Dodecandioic (C) N.D. N.D. N.D. -1.2 -0.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days N.D. N.D. N.D. -0.6 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. N.D. -1.0 -0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB N.D. N.D. N.D. +0.2 -1.0
supernatant control Vogels_fungal_media 0.1X N.D. N.D. N.D. +0.1 -0.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. N.D. -0.8 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. N.D. -0.8 +0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. N.D. -0.7 +0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X N.D. N.D. N.D. +0.4 -0.5
phage P. phyto PHYTO2 MOI 1 N.D. N.D. N.D. -0.7 +0.6
phage 24hr PHYTO11 MOI 0.1 N.D. N.D. N.D. +0.6 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. N.D. +0.8 -0.2
nutrient L-Serine N.D. N.D. N.D. +0.8 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. N.D. +0.6 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=15_days N.D. N.D. N.D. +0.5 +0.5
nophagecontrol 24hr Phyto Lib Only N.D. N.D. N.D. +0.5 +0.5
phage 24hr PHYTO4 MOI 10 N.D. N.D. N.D. +0.4 +0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. N.D. N.D. +0.6 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. +0.7 +0.5
phage 24hr PHYTO1 MOI 1 N.D. N.D. N.D. +0.7 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. N.D. N.D. +1.4 +0.0
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. N.D. N.D. +0.2 +1.2
carbon source Malonic (C) N.D. N.D. N.D. +1.1 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. N.D. N.D. +2.6 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. N.D. N.D. +2.7 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. N.D. N.D. +3.0 +0.1
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BPHYT_RS32580
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BPHYT_RS32585
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BPHYT_RS32600
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