Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS32420 and BPHYT_RS32425 are separated by 35 nucleotidesBPHYT_RS32425 and BPHYT_RS32430 are separated by 111 nucleotidesBPHYT_RS32430 and BPHYT_RS32435 overlap by 14 nucleotidesBPHYT_RS32435 and BPHYT_RS32440 are separated by 9 nucleotides BPHYT_RS32420: BPHYT_RS32420 - selenocysteine synthase, at 2,846,390 to 2,847,820 _RS32420 BPHYT_RS32425: BPHYT_RS32425 - formate dehydrogenase accessory protein FdhE, at 2,847,856 to 2,848,851 _RS32425 BPHYT_RS32430: BPHYT_RS32430 - formate dehydrogenase-N subunit gamma, at 2,848,963 to 2,849,619 _RS32430 BPHYT_RS32435: BPHYT_RS32435 - formate dehydrogenase-N subunit beta, at 2,849,606 to 2,850,538 _RS32435 BPHYT_RS32440: BPHYT_RS32440 - formate dehydrogenase, at 2,850,548 to 2,852,977 _RS32440
Group Condition BPHYT_RS32420 BPHYT_RS32425 BPHYT_RS32430 BPHYT_RS32435 BPHYT_RS32440
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 -0.8 -0.8 -0.3 -0.2
carbon source Phenylacetic 5 mM (C) -0.6 -0.5 -0.4 -0.5 -0.4
carbon source Phenylacetic 5 mM (C) -0.6 -1.0 -0.0 -0.2 -0.3
carbon source Sebacic (C) +0.1 -0.3 -0.4 -0.9 -0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +0.1 -0.6 +0.3 -0.8 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.2 -0.7 -0.1 -0.6 +0.0
carbon source 2-Deoxy-D-ribonic lithium salt (C) -0.0 -0.4 -0.9 +0.3 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 -0.5 -0.7 +0.2 -0.1
phage 36hr PHYTO2 MOI 1 -0.5 -0.4 +0.2 +0.2 -0.3
phage 24hr PHYTO6 MOI 1 -0.1 -0.5 -0.6 +1.0 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.5 -0.8 +0.3 -0.1 +0.2
stress Paraquat dichloride 0.064 mg/ml +0.3 -1.1 -0.0 +0.7 +0.2
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -0.9 +0.3 +0.2 +0.4 +0.2
phage 36hr PHYTO11 MOI 0.1 +0.2 -0.5 +0.0 +0.7 -0.4
nophagecontrol 36hr Phyto Lib Only -0.0 -0.5 +0.2 +0.7 -0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 +0.1 -0.4 +0.8 -0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.6 +0.8 -0.4 -0.8 +0.0
phage 36hr PHYTO8 MOI 10 -0.5 +0.1 +0.3 +0.5 -0.2
nophagecontrol 36hr Phyto Lib only +0.4 -0.3 -0.1 +0.8 -0.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.4 -0.4 +0.2 +0.7 -0.1
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.0 +0.4 -0.4 +0.8 +0.1
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.4 -0.0 -0.1 +1.5 -0.1
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X -0.3 +0.4 +0.3 +0.7 -0.1
phage 24hr PHYTO2 MOI 0.1 -0.3 +0.1 +0.3 +0.9 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 -0.1 -0.1 +1.1 -0.1
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) +0.6 -0.4 +0.2 +0.5 +0.4
motility outer cut, LB soft agar motility assay +0.1 +0.3 +0.0 +1.1 -0.1
motility outer cut, LB soft agar motility assay +0.4 +0.1 +0.2 +0.9 -0.1
phage P. phyto PHYTO2 MOI 1 +0.6 +0.7 +0.4 -0.3 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.4 +0.3 -0.2 +1.2 -0.0
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