Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS32355 and BPHYT_RS32360 overlap by 14 nucleotidesBPHYT_RS32360 and BPHYT_RS32365 are separated by 72 nucleotidesBPHYT_RS32365 and BPHYT_RS32370 are separated by 323 nucleotidesBPHYT_RS32370 and BPHYT_RS32385 are separated by 2840 nucleotides BPHYT_RS32355: BPHYT_RS32355 - ABC transporter permease, at 2,830,173 to 2,831,045 _RS32355 BPHYT_RS32360: BPHYT_RS32360 - spermidine/putrescine ABC transporter ATPase, at 2,831,032 to 2,832,120 _RS32360 BPHYT_RS32365: BPHYT_RS32365 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,832,193 to 2,833,260 _RS32365 BPHYT_RS32370: BPHYT_RS32370 - LysR family transcriptional regulator, at 2,833,584 to 2,834,501 _RS32370 BPHYT_RS32385: BPHYT_RS32385 - integrase, at 2,837,342 to 2,838,652 _RS32385
Group Condition BPHYT_RS32355 BPHYT_RS32360 BPHYT_RS32365 BPHYT_RS32370 BPHYT_RS32385
phage 36hr PHYTO8 MOI 0.1 N.D. -4.1 -0.1 -0.6 -0.2
phage 36hr PHYTO2 MOI 0.1 N.D. -4.2 +0.1 -0.6 +0.2
nophagecontrol 36hr Phyto Lib Only N.D. -4.5 -0.1 +0.3 +0.0
phage 24hr PHYTO7 MOI 10 N.D. -3.7 +0.2 -0.4 -0.2
phage 36hr PHYTO3 MOI 1 N.D. -2.9 +0.0 +0.0 +0.1
phage 24hr PHYTO3 MOI 10 N.D. -1.4 -0.7 -0.3 -0.2
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. -2.3 -0.0 -0.2 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -2.4 -0.6 +0.3 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -1.2 -1.5 +0.6 -0.1
phage 36hr PHYTO10 MOI 1 N.D. -1.8 +0.0 -0.3 -0.2
phage 24hr PHYTO6 MOI 0.1 N.D. -2.0 +0.1 -0.0 -0.2
phage 24hr PHYTO10 MOI 10 N.D. -2.7 +0.3 +0.2 +0.2
phage 24hr PHYTO MOI 0.1 N.D. -1.4 -0.7 -0.1 +0.2
phage 24hr PHYTO8 MOI 1 N.D. -1.3 -0.4 -0.4 +0.1
phage 36hr PHYTO4 MOI 1 N.D. -1.8 -0.5 +0.3 +0.1
lb_dap plate control LB_DAP agar plate, outgrowth in LB N.D. -1.1 -0.8 +0.1 +0.2
phage 24hr PHYTO4 MOI 10 N.D. -3.5 +1.4 +0.5 -0.0
phage P. phyto corn MOI 1 N.D. -1.3 -0.6 +0.3 +0.1
phage P. phyto PHYTO2 MOI 1 N.D. -1.5 -0.8 +0.5 +0.4
phage 24hr PHYTO1 MOI 10 N.D. -1.5 +0.4 -0.2 +0.0
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. -1.7 -0.1 +0.2 +0.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. -1.2 -0.8 +0.7 +0.2
nophagecontrol 24hr Phyto Lib only N.D. -1.8 +0.7 +0.1 +0.0
phage 24hr PHYTO10 MOI 10 N.D. -2.0 +0.6 +0.5 -0.1
vitamin With vitamins t1 N.D. +0.6 -1.0 -0.5 +0.1
phage 36hr PHYTO2 MOI 1 N.D. -1.5 +1.2 -0.4 +0.1
phage 36hr PHYTO3 MOI 1 N.D. -1.2 +0.6 +0.2 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +1.3 -0.1 -0.6 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.6 -0.6 +0.7 +0.3
phage 24hr PHYTO11 MOI 0.1 N.D. +1.7 +1.0 +0.2 +0.0
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