Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS32300 and BPHYT_RS32305 are separated by 202 nucleotidesBPHYT_RS32305 and BPHYT_RS32310 are separated by 154 nucleotidesBPHYT_RS32310 and BPHYT_RS32315 are separated by 181 nucleotidesBPHYT_RS32315 and BPHYT_RS32320 overlap by 4 nucleotides BPHYT_RS32300: BPHYT_RS32300 - integrase, at 2,816,092 to 2,817,609 _RS32300 BPHYT_RS32305: BPHYT_RS32305 - hypothetical protein, at 2,817,812 to 2,818,234 _RS32305 BPHYT_RS32310: BPHYT_RS32310 - porin, at 2,818,389 to 2,819,576 _RS32310 BPHYT_RS32315: BPHYT_RS32315 - FAD-dependent oxidoreductase, at 2,819,758 to 2,820,882 _RS32315 BPHYT_RS32320: BPHYT_RS32320 - nopaline dehydrogenase, at 2,820,879 to 2,822,294 _RS32320
Group Condition BPHYT_RS32300 BPHYT_RS32305 BPHYT_RS32310 BPHYT_RS32315 BPHYT_RS32320
phage 24hr PHYTO1 MOI 1 N.D. N.D. -1.6 N.D. -0.4
phage 24hr PHYTO10 MOI 10 N.D. N.D. -1.4 N.D. -0.5
phage 24hr PHYTO2 MOI 1 N.D. N.D. -0.9 N.D. -0.8
phage 36hr PHYTO6 MOI 0.1 N.D. N.D. -1.0 N.D. -0.5
phage 36hr PHYTO11 MOI 0.1 N.D. N.D. -0.7 N.D. -0.7
phage 36hr PHYTO3 MOI 0.1 N.D. N.D. -0.7 N.D. -0.6
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. N.D. -0.9 N.D. -0.4
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. N.D. -0.9 N.D. -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -0.9 N.D. -0.3
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. N.D. -1.1 N.D. -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -0.8 N.D. -0.2
phage 24hr PHYTO MOI 0.1 N.D. N.D. -0.8 N.D. -0.2
phage 24hr PHYTO1 MOI 10 N.D. N.D. -0.6 N.D. -0.4
phage 24hr PHYTO11 MOI 1 N.D. N.D. -0.8 N.D. -0.2
nophagecontrol 24hr Phyto Lib Only N.D. N.D. -0.6 N.D. -0.3
phage 24hr PHYTO4 MOI 10 N.D. N.D. -0.7 N.D. -0.3
phage 24hr PHYTO MOI 0.1 N.D. N.D. -0.6 N.D. -0.3
phage 36hr PHYTO3 MOI 1 N.D. N.D. -0.8 N.D. -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -0.8 N.D. -0.1
phage 36hr PHYTO1 MOI 10 N.D. N.D. -0.9 N.D. +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -0.8 N.D. +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.4 N.D. -0.9
phage 24hr PHYTO7 MOI 10 N.D. N.D. -0.7 N.D. +0.4
lb_dap plate control LB_DAP agar plate, outgrowth in LB N.D. N.D. -0.5 N.D. +0.4
phage 36hr PHYTO11 MOI 1 N.D. N.D. -0.5 N.D. +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -0.5 N.D. +0.4
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.4 N.D. -0.5
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. N.D. +0.8 N.D. -0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. +1.5 N.D. -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. +0.3 N.D. +1.3
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