Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS32000 and BPHYT_RS32005 are separated by 98 nucleotidesBPHYT_RS32005 and BPHYT_RS32010 are separated by 100 nucleotidesBPHYT_RS32010 and BPHYT_RS32015 overlap by 4 nucleotidesBPHYT_RS32015 and BPHYT_RS32020 overlap by 4 nucleotides BPHYT_RS32000: BPHYT_RS32000 - integrase, at 2,739,569 to 2,741,005 _RS32000 BPHYT_RS32005: BPHYT_RS32005 - hypothetical protein, at 2,741,104 to 2,741,682 _RS32005 BPHYT_RS32010: BPHYT_RS32010 - DEAD/DEAH box helicase, at 2,741,783 to 2,744,374 _RS32010 BPHYT_RS32015: BPHYT_RS32015 - hypothetical protein, at 2,744,371 to 2,746,176 _RS32015 BPHYT_RS32020: BPHYT_RS32020 - hypothetical protein, at 2,746,173 to 2,747,369 _RS32020
Group Condition BPHYT_RS32000 BPHYT_RS32005 BPHYT_RS32010 BPHYT_RS32015 BPHYT_RS32020
supernatant control Vogels_fungal_media; pH 5.5 N.D. N.D. -0.6 -0.1 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. -0.2 -0.3 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. N.D. -0.3 +0.2 -0.6
nitrogen source Ammonium chloride (N) N.D. N.D. +0.1 +0.1 -0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X N.D. N.D. -0.3 -0.4 +0.3
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) N.D. N.D. -0.2 +0.2 -0.4
phage 24hr PHYTO6 MOI 10 N.D. N.D. -0.4 -0.1 +0.3
supernatant control Vogels_fungal_media 0.1X N.D. N.D. -0.3 -0.1 +0.3
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -0.4 -0.2 +0.4
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. N.D. +0.4 -0.1 -0.3
phage 36hr PHYTO10 MOI 10 N.D. N.D. -0.4 +0.0 +0.3
phage 36hr PHYTO3 MOI 1 N.D. N.D. +0.3 -0.5 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. +0.3 +0.3 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. N.D. +0.2 +0.3 -0.3
carbon source L-Fucose (C) N.D. N.D. +0.3 +0.1 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB N.D. N.D. -0.3 +0.2 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. N.D. +0.3 +0.3 -0.4
phage P. phyto PHYTO2 MOI 1 N.D. N.D. -0.3 -0.0 +0.7
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.2 -0.2 +0.4
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.2 -0.2 +0.4
phage 36hr PHYTO9 MOI 0.1 N.D. N.D. -0.0 +0.2 +0.7
nophagecontrol 24hr Phyto Lib Only N.D. N.D. +0.1 +0.4 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. +0.3 +0.3 +0.3
r2a control R2A control N.D. N.D. +0.3 +0.2 +0.4
nophagecontrol 24hr Phyto Lib only N.D. N.D. +0.0 +0.2 +0.6
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. N.D. +0.1 -0.2 +0.9
nophagecontrol 24hr Phyto Lib Only N.D. N.D. +0.0 +0.5 +0.4
r2a control R2A control N.D. N.D. +0.2 +0.4 +0.4
r2a control R2A control N.D. N.D. +0.2 +0.5 +0.3
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. N.D. +0.5 +0.2 +0.3
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