Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS31945 and BPHYT_RS31950 are separated by 51 nucleotidesBPHYT_RS31950 and BPHYT_RS31955 are separated by 614 nucleotidesBPHYT_RS31955 and BPHYT_RS31960 are separated by 206 nucleotidesBPHYT_RS31960 and BPHYT_RS31965 are separated by 98 nucleotides BPHYT_RS31945: BPHYT_RS31945 - amino acid ABC transporter substrate-binding protein, at 2,726,400 to 2,727,593 _RS31945 BPHYT_RS31950: BPHYT_RS31950 - short-chain dehydrogenase, at 2,727,645 to 2,728,400 _RS31950 BPHYT_RS31955: BPHYT_RS31955 - 3-hydroxyisobutyrate dehydrogenase, at 2,729,015 to 2,729,917 _RS31955 BPHYT_RS31960: BPHYT_RS31960 - hypothetical protein, at 2,730,124 to 2,731,071 _RS31960 BPHYT_RS31965: BPHYT_RS31965 - mandelate racemase, at 2,731,170 to 2,732,303 _RS31965
Group Condition BPHYT_RS31945 BPHYT_RS31950 BPHYT_RS31955 BPHYT_RS31960 BPHYT_RS31965
phage 36hr PHYTO4 MOI 1 -0.7 -1.7 -0.1 -0.1 -0.3
phage 24hr PHYTO6 MOI 10 +0.1 -2.2 +0.1 -1.0 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -2.2 -0.2 -0.2 -0.0
phage 24hr PHYTO1 MOI 10 -0.0 -1.6 +0.3 -0.5 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.7 -1.8 -0.4 +0.9 -0.3
phage 24hr PHYTO6 MOI 0.1 -0.6 -1.1 -0.1 +0.2 -0.3
phage 36hr PHYTO1 MOI 1 -0.3 -1.3 +0.0 +0.4 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.4 -0.9 +0.2 -0.8 -0.2
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.1 -0.5 +0.4 -1.0 -0.3
phage 36hr PHYTO6 MOI 0.1 -0.1 -1.2 -0.1 +0.7 -0.4
motility inner cut, LB soft agar motility assay +0.2 -2.0 +0.3 +0.4 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.3 -1.5 -0.1 +0.4 -0.1
motility outer cut, LB soft agar motility assay +0.0 -1.7 +0.3 +0.4 -0.1
phage 24hr PHYTO9 MOI 0.1 -0.4 +0.8 +0.0 -0.8 -0.5
nitrogen source L-Isoleucine (N) -0.8 +0.7 +0.2 -0.8 -0.0
nitrogen source NAG (N) +0.0 -1.0 -0.2 +0.8 -0.3
motility outer cut, LB soft agar motility assay -0.2 -1.5 +0.1 +0.9 +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -1.7 +0.5 +0.6 +0.1 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.1 +1.4 -0.3 -0.9 -0.1
phage 36hr PHYTO3 MOI 0.1 -0.6 +0.5 -0.1 +0.8 -0.4
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.2 +1.1 +0.1 -0.7 -0.1
motility inner cut, LB soft agar motility assay -0.1 -0.6 +0.3 +1.3 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.6 +2.0 -0.3 -1.3 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.1 +1.4 +0.5 -0.5 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 +1.9 -0.0 -0.4 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 -0.5 +0.2 +0.9 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 +1.4 -0.3 +0.3 -0.2
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -0.1 +0.9 -0.1 +1.0 -0.3
phage 36hr PHYTO1 MOI 10 +0.4 +0.4 +0.1 +1.2 -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 +2.3 +0.0 -0.2 -0.2
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