Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS31760 and BPHYT_RS31765 are separated by 15 nucleotidesBPHYT_RS31765 and BPHYT_RS31770 are separated by 354 nucleotidesBPHYT_RS31770 and BPHYT_RS31780 are separated by 2008 nucleotidesBPHYT_RS31780 and BPHYT_RS31785 are separated by 433 nucleotides BPHYT_RS31760: BPHYT_RS31760 - aryldialkylphosphatase, at 2,681,354 to 2,682,403 _RS31760 BPHYT_RS31765: BPHYT_RS31765 - glutamate-1-semialdehyde 2,1-aminomutase, at 2,682,419 to 2,683,750 _RS31765 BPHYT_RS31770: BPHYT_RS31770 - TetR family transcriptional regulator, at 2,684,105 to 2,684,884 _RS31770 BPHYT_RS31780: BPHYT_RS31780 - indoleacetamide hydrolase, at 2,686,893 to 2,688,293 _RS31780 BPHYT_RS31785: BPHYT_RS31785 - hypothetical protein, at 2,688,727 to 2,688,957 _RS31785
Group Condition BPHYT_RS31760 BPHYT_RS31765 BPHYT_RS31770 BPHYT_RS31780 BPHYT_RS31785
phage 24hr PHYTO3 MOI 10 -2.1 -0.3 -0.9 -0.4 +0.1
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.4 +0.2 -1.6 -0.2 -0.7
phage P. phyto PHYTO2 MOI 0.1 -0.3 -0.3 -1.3 -0.1 -0.6
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.6 +0.1 -0.8 -0.8 -0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 -0.7 -0.9 +0.1 -0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.0 -0.3 -1.2 +0.4 -0.1
phage 36hr PHYTO8 MOI 0.1 -0.6 -0.5 -1.1 +0.0 +0.2
phage 24hr PHYTO9 MOI 10 -1.2 -0.5 -0.4 -0.4 +0.6
phage 24hr PHYTO4 MOI 0.1 -1.5 -0.4 -0.3 +0.4 -0.0
phage 24hr PHYTO6 MOI 0.1 -1.4 -0.3 -0.4 +0.4 -0.0
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.9 -0.4 -1.0 +0.4 +0.3
phage 36hr PHYTO4 MOI 0.1 -1.0 +0.3 -1.3 +0.1 +0.3
phage 36hr PHYTO6 MOI 0.1 -1.0 -0.3 -0.7 +0.3 +0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 -0.2 -0.9 +0.5 -0.8
nitrogen source L-Citrulline (N) +0.6 +0.1 -1.3 -0.1 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.9 -0.4 -1.4 +0.3 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.1 +0.4 -0.6 +0.4 +0.1
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -1.0 +0.1 +0.0 +0.8 -0.5
phage 36hr PHYTO4 MOI 1 -1.3 +0.0 -0.1 +0.8 +0.2
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days +0.5 +0.0 -0.7 +0.5 -0.7
phage 36hr PHYTO6 MOI 10 -0.8 -0.4 -0.4 +0.7 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.3 +0.4 +0.3 +0.6 +0.1
phage 36hr PHYTO7 MOI 0.1 -0.8 -0.2 +0.3 -0.1 +1.1
nitrogen source Putrescine (N) +0.6 +0.5 -0.6 +0.5 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +1.4 +0.1 -0.9 -0.2 +0.1
nophagecontrol 36hr Phyto Lib only -0.7 -0.2 +0.9 +0.1 +0.6
nophagecontrol 24hr Phyto Lib Only -0.6 +0.7 -0.4 +0.4 +0.7
carbon source L-Fucose (C) +0.8 -0.2 -0.7 +0.6 +0.3
phage 36hr PHYTO1 MOI 1 -0.7 +0.0 +0.3 +0.7 +0.7
phage 36hr PHYTO7 MOI 1 -0.7 +0.4 -0.3 +0.9 +1.0
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