Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS31735 and BPHYT_RS31740 are separated by 203 nucleotidesBPHYT_RS31740 and BPHYT_RS31745 are separated by 14 nucleotidesBPHYT_RS31745 and BPHYT_RS31750 are separated by 18 nucleotidesBPHYT_RS31750 and BPHYT_RS31755 overlap by 1 nucleotides BPHYT_RS31735: BPHYT_RS31735 - hydroxypyruvate isomerase, at 2,676,634 to 2,677,422 _RS31735 BPHYT_RS31740: BPHYT_RS31740 - ABC transporter permease, at 2,677,626 to 2,678,576 _RS31740 BPHYT_RS31745: BPHYT_RS31745 - ABC transporter ATP-binding protein, at 2,678,591 to 2,679,760 _RS31745 BPHYT_RS31750: BPHYT_RS31750 - ABC transporter, at 2,679,779 to 2,680,555 _RS31750 BPHYT_RS31755: BPHYT_RS31755 - amino acid ABC transporter ATPase, at 2,680,555 to 2,681,268 _RS31755
Group Condition BPHYT_RS31735 BPHYT_RS31740 BPHYT_RS31745 BPHYT_RS31750 BPHYT_RS31755
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.4 -0.8 -1.0 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.2 -0.8 -0.5 -0.3
r2a control R2A control N.D. -0.5 -0.6 +0.2 -0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.0 -0.9 +0.4 -0.8
phage 24hr PHYTO10 MOI 10 N.D. -0.6 -0.9 +0.5 +0.6
carbon source Azelaic (C) N.D. -0.3 +0.6 +0.2 -0.8
nophagecontrol 36hr Phyto Lib only N.D. -0.2 -1.1 +0.3 +0.8
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. -0.8 +0.1 -0.2 +0.8
phage 36hr PHYTO8 MOI 10 N.D. -0.4 +0.5 +0.8 -0.9
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 N.D. -0.4 +0.1 -0.4 +0.7
phage 24hr PHYTO2 MOI 1 N.D. -1.1 +0.0 +0.3 +0.8
phage 24hr PHYTO10 MOI 1 N.D. -0.5 -0.3 +0.3 +0.6
phage 24hr PHYTO4 MOI 0.1 N.D. -0.6 -0.5 +0.5 +0.8
phage 36hr PHYTO7 MOI 0.1 N.D. -0.3 -0.6 +0.6 +0.4
carbon source adipate (C) N.D. -0.3 +0.4 +0.7 -0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB N.D. -0.2 +0.6 +0.6 -0.4
supernatant control Vogels_fungal_media 0.1X N.D. +0.2 +0.6 +0.7 -0.7
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. +0.1 +0.7 +0.7 -0.6
phage 36hr PHYTO MOI 0.1 N.D. -0.5 +0.0 +0.5 +0.9
phage 24hr PHYTO1 MOI 10 N.D. -0.6 +0.3 +0.5 +0.8
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) N.D. -0.5 +0.5 -0.4 +1.3
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. +0.5 -0.3 -0.5 +1.2
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. +0.4 -0.5 +0.1 +1.0
nophagecontrol 24hr Phyto Lib Only N.D. -0.3 +0.5 +0.3 +0.7
phage 24hr PHYTO4 MOI 10 N.D. -0.1 -0.3 +0.3 +1.4
phage 36hr PHYTO2 MOI 1 N.D. -0.3 +0.6 +0.6 +0.5
phage P. phyto PHYTO2 MOI 1 N.D. +0.1 -0.1 +0.6 +1.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X N.D. +0.4 +0.5 +0.4 +0.5
phage 36hr PHYTO6 MOI 1 N.D. -0.3 +0.7 +0.4 +1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.0 +0.6 +0.3 +1.0
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