Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS31705 and BPHYT_RS31710 are separated by 123 nucleotidesBPHYT_RS31710 and BPHYT_RS31715 are separated by 133 nucleotidesBPHYT_RS31715 and BPHYT_RS31720 are separated by 362 nucleotidesBPHYT_RS31720 and BPHYT_RS31725 are separated by 190 nucleotides BPHYT_RS31705: BPHYT_RS31705 - MFS transporter, at 2,669,621 to 2,670,934 _RS31705 BPHYT_RS31710: BPHYT_RS31710 - porin, at 2,671,058 to 2,672,203 _RS31710 BPHYT_RS31715: BPHYT_RS31715 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,672,337 to 2,673,479 _RS31715 BPHYT_RS31720: BPHYT_RS31720 - LysR family transcriptional regulator, at 2,673,842 to 2,674,771 _RS31720 BPHYT_RS31725: BPHYT_RS31725 - hypothetical protein, at 2,674,962 to 2,675,183 _RS31725
Group Condition BPHYT_RS31705 BPHYT_RS31710 BPHYT_RS31715 BPHYT_RS31720 BPHYT_RS31725
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.2 +0.1 -1.3 -0.9 -0.6
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.4 -0.4 -1.0 -0.1 -0.6
phage 36hr PHYTO11 MOI 0.1 -0.2 -0.1 -0.7 -0.2 -1.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 -0.8 -1.0 +0.2 -0.5
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -0.0 -0.2 -1.7 -1.1 +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.1 -0.1 -1.0 -0.3 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=15_days +0.1 -0.4 -0.2 -0.5 -0.8
phage 24hr PHYTO1 MOI 10 +0.0 +0.2 -0.3 -0.5 -1.2
phage 36hr PHYTO7 MOI 1 +0.2 -0.6 -0.2 -0.1 -1.1
phage 36hr PHYTO9 MOI 0.1 -0.2 -0.4 +0.2 -0.5 -0.9
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 +0.2 -0.2 -0.9 -0.4 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.3 +0.1 -0.7 +0.2 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.5 -0.4 -0.5 +0.1 -0.5
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.2 -0.2 +0.3 +0.4 -1.0
nitrogen source L-Serine (N) -0.6 -0.1 +0.2 -0.5 +0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 +0.4 -0.1 -0.9 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 -0.1 +0.6 -0.3 -0.6
phage 36hr PHYTO10 MOI 10 +0.1 +0.2 +0.3 +0.5 -0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.2 -0.1 -0.6 +0.8 +0.4
carbon source propionate (C) -0.3 +0.3 +0.4 +0.9 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 +0.4 +0.8 -0.5 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 +0.1 -0.0 +1.7 -0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 -0.2 +0.6 +1.2 -0.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days +0.3 +0.0 +1.0 +0.4 -0.3
phage 24hr PHYTO7 MOI 10 -0.1 +0.5 +0.7 -0.2 +0.6
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.4 -0.2 +0.8 +0.5 +0.0
carbon source Malonic (C) +0.4 +0.3 +0.4 -0.3 +0.9
carbon source Phenylacetic 5 mM (C) +0.3 +0.7 -0.1 +0.5 +0.6
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 +0.1 +0.1 +0.8 +1.3
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.3 +0.6 +1.4 +0.3 +0.4
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